| 亚洲栽培稻遗传变异分析最少SSR引物数的研究 |
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| 引用本文： | 袁筱萍,王彩红,邓宏中,徐群,冯跃,余汉勇,王一平,魏兴华. 亚洲栽培稻遗传变异分析最少SSR引物数的研究[J]. 中国水稻科学, 2015, 29(6): 578-586. DOI: 10.3969/j.issn.1001G7216.2015.06.003 |
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| 作者姓名： | 袁筱萍 王彩红 邓宏中 徐群 冯跃 余汉勇 王一平 魏兴华 |
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| 作者单位： | 中国水稻研究所 水稻生物学国家重点实验室, 杭州 310006 |
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| 基金项目： | 科技部科技支撑计划资助项目(2013BAD01B02)农业部物种资源保护专项(2014NWB031) |
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| 摘 要： | 以69份类型明确的亚洲栽培稻品种为材料,选用120对SSR引物,通过评估遗传多样性和群体结构,研究分析其遗传变异对SSR引物数的要求。结果表明:1)120对引物在69份试验材料中共检测到1256个等位变异,每个位点的等位基因数差异较大,变化范围为2~27,平均为10.5;平均Nei基因多样性指数变化范围为0.4058~0.9452,平均为0.7616;2)分析亚洲栽培稻遗传多样性时,至少需要72对SSR引物;3)分析亚洲栽培稻群体结构时所需最少引物数可降低至60对。
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| 关 键 词： | 亚洲栽培稻,SSR标记 ,遗传多样性,群体结构,矩阵相关性检测, |
| 收稿时间： | 2015-01-19 |
| 修稿时间： | 2015-09-17 |
Minimum of SSR Markers for Analyzing Genetic Variation of Oryza sativa L. |
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| Xiao-ping YUAN,Cai-hong WANG,Hong-zhong DENG,Qun XU,Yue FENG,Han-yong YU,Yi-ping WANG,Xing-hua WEI. Minimum of SSR Markers for Analyzing Genetic Variation of Oryza sativa L.[J]. Chinese Journal of Rice Science, 2015, 29(6): 578-586. DOI: 10.3969/j.issn.1001G7216.2015.06.003 |
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| Authors: | Xiao-ping YUAN Cai-hong WANG Hong-zhong DENG Qun XU Yue FENG Han-yong YU Yi-ping WANG Xing-hua WEI |
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| Affiliation: | State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China |
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| Abstract: | A total of 120 SSR markers were used to determine the minimum of SSR markers for analyzing genetic variation of 69 Oryza sativa L. accessions, which have been clearly classified, based on the genetic diversity and population structure. The results showed that: 1) a total of 1,256 alleles were detected with 120 SSR markers. The number of alleles per locus widely ranged from 2 to 27 with an average of 10.5. Nei's genetic diversity index per locus varied from 0.413 to 0.951 with a mean of 0.760; 2) the minimum of SSR markers for analyzing genetic diversity of rice was 72; 3) the number of SSR markers for analyzing population structure of rice was at least 60. |
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| Keywords: | Oryza sativa L. simple sequence repeat genetic diversity population structure Mantel correlation test |
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