合成脂肪酶的影响

研究了碳源和氮源对白腐菌Sarcodon asparatus合成脂肪酶的影响。橄榄油是脂肪酶合成的最佳碳源，脂肪酶活力达0．65U／mL。蛋白胨、尿素和氯化铵对脂肪酶的合成有促进作用，它们的酶活力分别为0．65、0．67和0．62U／mL，而硫酸铵抑制脂肪酶的合成，其酶活力仅为0．08U／mL。C／N比对细胞生长和酶的合成影响很大，当C／N比从2增加到10时，脂肪酶活力从0．65下降到0．14U／mL，而细胞浓度从3．08升高到4．58mg／mL。在培养基初始pH值5．5、28℃下培养，S．asparatus合成的脂肪酶活力最高，脂肪酶活力和细胞浓度分别为0．65U／mL和3．08mg／mL。

EFFECTS OF CARBON AND NITROGEN SOURCES ON LIPASE PRODUCTION BY SARCODON ASPARATUS

Carbon sources and nitrogen sources affecting lipase production by Sarcodon asparatus were investigated.Lipase activity was the highest(0.65 U/mL) for olive oil compared with other carbon sources.Lipase production was stimulated by peptone(0.65 U/mL),urea(0.67 U/mL) and ammonium chloride(0.62 U/mL),and repressed by ammonium sulfate with a low lipase activity of 0.08 U/mL.C/N ratio was a significant factor for lipase production and cell growth.When C/N ratio was raised from 2 to 10,lipase activity decreased from 0.65 to 0.14 U/mL,and biomass increased from 3.08 to 4.58 mg/mL.The optimal culture conditions of S.asparatus were pH value 5.5 and 28 ℃,and lipase activity was 0.65 U/mL with a biomass concentration of 3.08 mg/mL.