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不同采收期刺五加叶中多糖含量测定
引用本文:张崇禧,黄建军,施威,高笑一,丛登立.不同采收期刺五加叶中多糖含量测定[J].人参研究,2008,20(1):12-15.
作者姓名:张崇禧  黄建军  施威  高笑一  丛登立
作者单位:1. 山东大学威海分校海洋学院,山东威海,264209
2. 吉林农业大学中药材学院,吉林长春,130118
3. 吉林大学药学院,吉林长春,130021
摘    要:目的为了对刺五加的质量进行综合评价。方法采用蒽酮-硫酸比色法,以葡萄糖作为对照品,在620nm波长下测定吸光度,分别测定刺五加叶中可溶性多糖和粗多糖的含量。结果在0.083mg-0.338mg范围内,葡萄糖mg数与吸光度A值线性关系良好,平均回收率为100.32%,RSD=0.223%,R2=0.9990。刺五加叶的部位可溶性多糖含量为1.770%,粗多糖含量为1.290%。结论不同采收期刺五加叶中多糖含量差别很大,9月份采收的苇沙河地区的刺五加叶总多糖含量较高。从方法学考察可看出,本方法简便、快速、可靠、易行、重现性好,适合刺五加多糖的含量测定。

关 键 词:刺五加    多糖  采收期  含量测定

Determinate the content ofpolysaccharidesin different collecting time of production of Acanthopanax senticosus
Authors:ZHANG Chong-xi  HUANG Jian-jun  SHI Wei  Gao Xiao-yi  Cong Deng-li
Institution:ZHANG Chong-xi HUANG Jian-jun(Marine College,Sh,ong University at WeihaiWeihai 264209)SHI Wei Gao Xiao-yi(College of Chinese Medicinal Material,Jilin Agricultural University,ChangChun,130118)Cong Deng-li(School of pharmacy,Jilin University,changchun,Jinlin,130041,China)
Abstract:ObjectIn order to comprehend evaluate the quality of Acanthopanax senticosus.To determinate the content of polysaccharidesin Acanthopanax senticosus.MethodsThe content of polysaccharidesin Acanthopanaxsenticosuswas determined by anthracene and byvitriol ofcolorimetry.Glucosewas wasstandard preparation.Wave length was 620nm.ResultsThe mass of glucose(mg)and Abs were linear in the range of 0.083mg-0.338mg.The average recovery was 100.32%,RSD=0.223%,R2=0.9990.The content ofpolysaccharidesof Acanthopanax sentic...
Keywords:Acanthopanax senticosus leaves  polysaccharides    
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