| 平邑甜茶叶盘组培再生研究初报 |
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| 引用本文： | 张军科,沈 向,曹庆芹.平邑甜茶叶盘组培再生研究初报[J].西北农林科技大学学报(社会科学版),2000,28(4):94-97. |
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| 作者姓名： | 张军科 沈 向 曹庆芹 |
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| 作者单位： | 1. 西北农林科技大学,园艺系,陕西,杨陵,712100
2. 山东农业大学,园艺系,山东,泰安,271018 |
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| 基金项目： | 西北农业大学青年教师科研基金资助项目 |
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| 摘 要： | 对平邑甜茶叶盘离体再生条件进行了研究 ,结果表明 ,平邑甜茶叶盘愈伤和分化适宜的 BA和 IBA质量浓度分别为 0 .5～ 1 .0和 0 .2 mg/L,愈伤至少需黑暗培养 35d;茎尖分化的适宜培养基为 MS附加 BA 0 .5mg/L、IBA 0 .1～ 0 .2 mg/L ;适宜的生根培养基为1 /2 MS附加 IBA0 .1 mg/L。
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| 关 键 词： | 平邑甜茶 组织培养 植株再生 叶盘法 |
| 修稿时间： | 1999-10-08 |
Study on the regeneration of Malus hupehensis Rehd. |
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| ZHANGJun-ke.Study on the regeneration of Malus hupehensis Rehd.[J].Journal of Northwest Sci-Tech Univ of Agr and,2000,28(4):94-97. |
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| Authors: | ZHANGJun-ke |
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| Institution: | 1\j (1 Department of Horticulture,Northwest Science and Technology University of Agriculture and Forestry,Yangling,Shaanxi 712100,China;2 Department of Horticulture,Shandong Agricult |
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| Abstract: | Conditions influencing regeneration by leaf disk of Pingyitiancha ( Malus hupehensis hd.) were studied.The result showed that the optimum concentrations of benzyladenine (BA) and Indole 3 butyric (IBA) for Pingyitiancha leaf disk callus inducing and differentiation were 0.5-1.0 mg/L and 0.2 mg/L respectively.35 days dark culture was needed during callus inducing.The optimum medium for shoot tip differentiation was MS supplemented with BA 0.5 mg/L and IBA 0.1-0.2 mg/L.The optimum medium for rooting was 1/2MS supplemented with IBA 0.1 mg/L. |
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| Keywords: | Malus hupehensis Rehd tissue culture plant regeneration leaf disk method |
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