| 转EPSPS基因大豆植株中蛋白的表达 |
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| 引用本文： | 邢珍娟,李飞武,刘娜,李葱葱,康岭生,宋新元,邵改革,张明.转EPSPS基因大豆植株中蛋白的表达[J].大豆科学,2009,28(6). |
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| 作者姓名： | 邢珍娟 李飞武 刘娜 李葱葱 康岭生 宋新元 邵改革 张明 |
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| 作者单位： | 吉林省农业科学院农业部转基冈植物环境安全监督检验测试中心,吉林,长春,130033 |
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| 基金项目： | 转基因生物新品种培育重大专项资助项目 |
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| 摘 要： | 采用ELISA定量测定法研究了转EPSPs基因大豆不同生长时期不同器官中CP4 EPSPS蛋白含量的变化.结果表明:转EPSPS基因大豆不同器官在不同牛育期CP4 EPSPS蛋白含量表现出较大的差异,R8期籽粒中的CP4EPSPS蛋白含量在所有时期和所有组织中蛋门含世最高;上位叶和下位叶中CP4 EPSPS蛋白除V3～V5期和R8期外的表达趋势一致,茎上部和茎下部中CP4 EPSPS蛋白在不同时期表达动态趋势基本一致,根中CP4 EPSPS蛋白含量存V3～V5期下降,然后逐渐升高,R1～R8期有一个大幅度的下降过程.从V1期至R8期,随着植株的不断生长,各组织中CP4 EPSPS蛋白的含量有明显的变化.
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| 关 键 词： | 转基因大豆 CP4 EPSPE蛋白含量 酶联免疫吸附测定 |
Expression of CP4 EPSPS Protein of Genetically Modified Roundup Ready Soybean |
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| XING Zhen-juan,LI Fei-wu,LIU Na,LI Cong-cong,KANG Ling-sheng,SONG Xin-yuan,SHAO Gai-ge,ZHANG Ming.Expression of CP4 EPSPS Protein of Genetically Modified Roundup Ready Soybean[J].Soybean Science,2009,28(6). |
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| Authors: | XING Zhen-juan LI Fei-wu LIU Na LI Cong-cong KANG Ling-sheng SONG Xin-yuan SHAO Gai-ge ZHANG Ming |
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| Abstract: | The content of CP4 EPSPS protein in transgenic soybean plant was studied by Elisa method in this paper. Results showed that significant difference of CP4 EPSPS protein contents existed in different tissues and growth stages,with CP4 EPSPS protein content in seeds at maturity the highest. The contents of CP4 EPSPS protein in the upper and the bottom leaves were not significantly different in all growth stages except stages V3-V5 and R8. The contents of CP4 EPSPS protein in the upper and the bottom stalks showed consistent trend in different stages. The content of CP4 EPSPS protein in root decreased in stage V3-V5, then increased gradually,and then decreased from R1 to R8. With the growth of the plant, the content of the protein of CP4 EPSPS in all tissues changed greatly from VI to V8 stage. |
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| Keywords: | Transgenic soybean CP4 EPSPS protein content Enzyme-linked immunosorbent assay |
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