12个梨品种S基因型的鉴定

应用PCR扩增技术, 根据目的S基因与GenBank中已知S 基因同源性100%原理鉴定了我国育成的12个梨品种的S基因型, 分别是: ‘红酥脆’为S₄S₃₆ , ‘新梨7号’为S₂₈S_d , ‘金水3号’为S₅S₂₉ , ‘八月酥’为S₃S₁₆ , ‘金水1号’为S₃S₂₉ , ‘龙泉酥’为S₃S₂₂ , ‘雪花’为S₄S₁₆ , ‘雪芳’为S₄S₁₆ ,‘雅青’为S₄S₃₄ , ‘新雅’为S₄S₃₄ , ‘德胜香’为S₃S₂₉ , ‘富源黄’为S₁₆S₃₃。通过对S基因的DNA序列分析, 确认S₁₆、S₃₁为同一S基因。本研究结果丰富了我国梨品种的S基因型信息, 为田间合理配置授粉品种提供了依据。

Identification of S-genotypes of Twelve Pear Cultivars by Analysis of DNA Sequence

Using PCR amplification method, S-genotypes of twelve Chinese pear cultivars were determined. The S-genotypes were S4S36 for Hongsucui, S28Sd for Xinli 7, S5S29 for Jinshui 3, S3S16 for Bayuesu, S3S29 for Jinshui 1, S3S22 for Longquansu, S4S16 for Xuehua, S4S16 for Xuefang, S4S34 for Yaqing, S4S34 for Xinya, S3S29 for Deshengxiang, S31S33 for Fuyuanhuang. Based on the analysis of the S gene, It was confirmed that S16 and S31 were the same S gene. The study results enriched the genotype information of pear cultivars in China, providing the basis for appropriate arrangement of pollination tree in pear orchards.