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| 大豆GmRAV基因的密码子偏好性分析 | |
| 引用本文： | 杨春亮,王良,武斌,赵琳,张晓丽,钟淑琦. 大豆GmRAV基因的密码子偏好性分析[J]. 东北农业大学学报, 2012, 0(7): 34-41 |
| 作者姓名： | 杨春亮 王良 武斌 赵琳 张晓丽 钟淑琦 |
| 作者单位： | 1. 哈尔滨医科大学基础医学院,哈尔滨150081 2. 东北农业大学大豆研究所,哈尔滨150030 3. 黑龙江生物科技职业学院食品系,哈尔滨150025 4. 哈尔滨市农业科学院,哈尔滨150070 |
| 基金项目： | 黑龙江省教育厅科技计划项目(11541181) |
| 摘 要： | 运用CUSP和CodonW程序分析大豆GmRAV基因的密码子偏好性,并与其他11种植物的RAV基因密码子偏好性进行比较,以期为该基因在作物遗传改良中选择合适的受体植物提供参考.结果表明,大豆GmRAV基因偏好于以C或G结尾的密码子,基于RAV基因的密码子使用偏性的系统聚类分析表明,大豆等共9种双子叶植物聚为一类,玉米、高粱和水稻这3种单子叶植物聚为一类,预示大豆GmRAV基因更适合导入双子叶植物 |
| 关 键 词： | 大豆 RAV基因 密码子偏好性 聚类分析 |
Codon bias analysis of soybean GmRAV gene |
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| YANG Chunliang,WANG Liang,WU Bin,ZHAO Lin,ZHANG Xiaoli,ZHONG Shuqi. Codon bias analysis of soybean GmRAV gene[J]. Journal of Northeast Agricultural University, 2012, 0(7): 34-41 | |
| Authors: | YANG Chunliang WANG Liang WU Bin ZHAO Lin ZHANG Xiaoli ZHONG Shuqi |
| Affiliation: | 1(1.Basic Medical Science School,Harbin Medical University,Harbin 150081,China;2.Soybean Research Institute,Northeast Agricultural University,Harbin 150030,China;3.Food Department,Heilongjiang Vocational School of Biology Science and Technology,Harbin 150025,China;4.Harbin Academy of Agricultural Science,Harbin 150070,China) |
| Abstract: | Coding sequences of RAV genes from soybean and other 11 species were analyzed by CUSP and CodonW programs for identifying codon bias and selecting appropriate expression systems.The results showed that soybean GmRAV gene was biased toward the synonymous codons with C and G at the third codon position.The phylogenic analysis suggested GmRAV was evolutionarily closer to nine dicotyledons than monocotyledons(Zea mays,Sorghum bicolor and Oryza sativa).The dicotyledons plant expression system may be more suitable for expression of soybean GmRAV gene. |
| Keywords: | soybean RAV gene codon bias cluster analysis |
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