桑树原生质体分离条件的优化

为了获得桑树原生质体分离的最优条件,为今后桑树原生质体融合、新种质的获取等植物遗传改良提供理论基础,采用酶解法和血球板计数法,对桑树原生质体分离的影响因素进行研究。结果表明,酶、酶液组合、酶解时间和渗透压稳定剂等都对原生质体的制备有显著的影响,较适宜桑树叶片游离的组合条件是1.0%纤维素酶+0.5%果胶酶+0.2%离析酶+0.6mol/L甘露醇+CPW盐溶液,酶解温度为28℃±2℃,酶解时间为6h。在此条件下可获得高产量的原生质体。

Optimization of Protoplast Isolation Condition in Mulberry

The factors affecting mulberry protoplast isolation were studied by the enzyme hydrolysis and hemacytometer methods to optimize the mulberry protoplast isolation condition and to provide the theoretical basis for mulberry protoplast fusion,new germplasm and plant genetic improvement.The results showed that enzyme type,different enzyme combination,enzymolysis time and osmotic pressure had significant effect on mulberry protoplast isolation.The higher mulberry protoplast yield could be obtained under the optimum condition including 1.0% cellulase,0.5% pectolase,0.2% macerozyme,0.6 mol/L mannitol,CPW,and 28 ℃ ±2 ℃ for 6 h.