| 柑橘种间体细胞杂种的核质遗传研究 |
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| 引用本文： | 徐小勇,刘继红.柑橘种间体细胞杂种的核质遗传研究[J].果树学报,2010(2). |
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| 作者姓名： | 徐小勇 刘继红 |
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| 作者单位： | 扬州大学园艺与植物保护学院;华中农业大学·作物遗传改良国家重点实验室; |
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| 基金项目： | 国家自然科学基金(编号:30200189) |
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| 摘 要： | 为了解柑橘体细胞杂种的核质遗传组成,采用流式细胞仪(FCM)、简单序列重复(SSR)、相关序列扩增多态性(SRAP)以及酶切扩增多态性序列(CAPS)对此前通过对称融合获得的2株种间体细胞杂种进行了分析。结果表明,在丹西红橘+红江橙种间组合中,分析的2株植株中1株为二倍体非对称体细胞杂种,其核DNA具有双亲的部分DNA,并有重组发生,胞质DNA则来源于红江橙(叶肉亲本);而另1株则是异源四倍体体细胞杂种,其核DNA来源于双亲,并有重组发生,胞质DNA则来源于红江橙。
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| 关 键 词： | 柑橘 体细胞杂种 FCM SSR SRAP CAPS |
Inheritance of nuclear and cytoplasmic components of inter-specific somatic hybrids in Citrus |
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| XU Xiao-yong,LIU Ji-hong.Inheritance of nuclear and cytoplasmic components of inter-specific somatic hybrids in Citrus[J].Journal of Fruit Science,2010(2). |
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| Authors: | XU Xiao-yong LIU Ji-hong |
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| Institution: | 1College of Horticulture and Plant Protection;Yangzhou University;Yangzhou;Jiangsu 225009 China;2National Key Laboratory of Crop Genetic Improvement;Huazhong Agricultural University;Wuhan;Hubei 430070 China |
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| Abstract: | FCM,SSR,SRAP and CAPS were employed to characterize the nuclear and cytoplasmic components of two inter-specific somatic hybrid plants obtained by symmetric fusion.The results showed that in Dancy tangerine(Citrus reticulata)+ sweet orange(C.sinensis Osbeck cv.Hongjiangcheng) inter-specific combination,one of two analyzed plants was diploid asymmetric somatic hybrid,which inherited nuclear DNA from some of both parents and cytoplasmic composition from sweet orange(leaf parent);the another one was tetraploid... |
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| Keywords: | Citrus Somatic hybrids FCM SSR CAPS SRAP |
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