加味柴胡汤对子宫内膜炎奶牛血液白细胞的影响

本试验选子宫内膜炎患牛10头,随机分为中药治疗组和患病对照组（每组5头）,5头健康奶牛作为正常对照组.中药治疗组子宫灌注复方中药剂加味柴胡汤,连用6d,其他两组给予生理盐水.在用药前1d和用药2d、4d、6d采血,用血液自动分析仪检测.结果：（1）治疗前,中药治疗组和患病对照组白细胞数量显著（P＜0.05）高于正常对照组,治疗6d后,中药治疗组显著（P＜0.05）低于治疗前和患病对照纽.（2）治疗前,中药治疗组和患病对照组中间细胞数量和比率均显著（P＜0.05）高于正常对照组,用药6d后,中药治疗组显著（P＜0.05）或极显著（P＜0.01）低于患病对照组和治疗前.（3）治疗前,中药治疗组和患病对照组嗜中性粒细胞数量比率显著（p＜0.05）高于正常对照组.用药6d后,中药治疗组与正常对照组均显著（P＜0.05）低于患病对照组,低于治疗前.（4）治疗前,中药治疗组和患病对照组淋巴细胞数量显著（P＜0.05）高于正常对照组,比率显著（P＜0.05）低于正常对照组.用药6d后,中药治疗组和正常对照组淋巴细胞数量和比率显著（P＜0.05）高于患病对照纽.结论：加味柴胡汤可降低子宫内膜炎奶牛的炎症反应,提高免疫力.

Effect of Modified Bupleurum Decoction on White Blood Cell of Endometritis in Dairy Cattle

A total of 15 cows （10 suffering dairy cows and 5 healthy cows） were selected in this study,and these 10 suffering dairy cows were divided into Chinese medicine treatment group and sick control group （5 cows in each group）.Another 5 healthy cows were in the normal control group.The suffering dairy cows treated with the modified bupleurum decoction for 6 days,sick control group and normal control group cows were not carried out any drug treatment during the test period.The jugular vein blood of dairy cows in each group were collected and determined in the pre-treatment and the 2d,4d,6d after the treatment with automatic blood analyzer.Results showed that：（1） white blood cell,intermediate cell,neutrophil and Lymphocyte of Chinese medicine treatment group and sick control group were higher significantly（P＜0.05）than that of normal control group in the pre-treatment.After six days of the treatment,white blood cell was lower in Chinese medicine treatment group than that of control group and in the pre-treatment obviously（P＜0.05）.（2）After six days of treatment,intermediate cell was lower in Chinese medicine treatment group than that of control group and in the pre-treatment obviously（P＜0.05 or P＜0.01）.（3）After six days of treatment,neutrophils was lower in Chinese medicine treatment group than that of control group and in the pretreatment obviously（P＜0.05 or P＜0.01）.（4）After six days of treatment,Lymphocyte was lower in Chinese medicine treatment group than that of control group and in the pre-treatment obviously（P＜0.05）.In a conclusion,the modified bupleurum decoction could inhibit inflammation and improve immunity.