| 管芯片技术通量检测杨树溃疡病病菌的应用 |
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| 引用本文： | 王凯英,高茜,严东辉. 管芯片技术通量检测杨树溃疡病病菌的应用[J]. 林业科学研究, 2016, 29(4): 615-619 |
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| 作者姓名： | 王凯英 高茜 严东辉 |
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| 作者单位： | 中国林业科学研究院森林生态环境与保护研究所 国家林业局森林保护学重点实验室, 北京 100091;中国林业科学研究院森林生态环境与保护研究所 国家林业局森林保护学重点实验室, 北京 100091;中国林业科学研究院森林生态环境与保护研究所 国家林业局森林保护学重点实验室, 北京 100091;南京林业大学南方现代林业协同创新中心, 南京 210037 |
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| 基金项目： | 水解纤维素产烃树木内生菌资源利用技术引进（2013-4-10）、效应物在杨盘二孢半活养方式及亲和寄主致病性中的作用（31370645） |
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| 摘 要： | 正基因芯片技术因能对生物样本进行快速、高通量的定性和定量分析,而在植物病害诊断中也得到普遍的应用[1]。树木溃疡病是指引起木本植物树皮上出现溃疡腐烂等症状的病害[2]。引起杨树溃疡病的真菌种类较多,加之其无性型和有性型多样,所以,关于杨树溃疡病病原菌的报道多有不同。另外,受地理位置、寄主植物关系及分类体系的限制,迫切需要发展不依赖于常规分离培养的病原鉴定新技术。
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| 关 键 词： | 溃疡病菌 Botryosphaeria dothidea 芯片杂交 检测 管芯片 |
| 收稿时间： | 2016-03-09 |
Detection of Poplar Canker Fungal Pathogens by ArrayTube Technique |
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| WANG Kai-ying,GAO Qian and YAN Dong-hui. Detection of Poplar Canker Fungal Pathogens by ArrayTube Technique[J]. Forest Research, 2016, 29(4): 615-619 |
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| Authors: | WANG Kai-ying GAO Qian YAN Dong-hui |
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| Affiliation: | Research Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry, Key Laboratory of Forest Protection of State Forestry Administration, Beijing 100091, China;Research Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry, Key Laboratory of Forest Protection of State Forestry Administration, Beijing 100091, China;Research Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry, Key Laboratory of Forest Protection of State Forestry Administration, Beijing 100091, China;Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, Jiangsu, China |
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| Abstract: | [Objective] Using ArrayTube, a high-throughput microarray technology, to detect poplar canker pathogens in Beijing area. [Method] The DNA extracted from symptomatic and asymptomatic poplar bark were used to hybridizing by ArrayTube. The results of hybridization were recorded and analyzed by IconoClust-AT 3.0. Then the hybridization results were verified by cloning of the corresponding tissue. [Result] The results showed that Botryosphaeria dothidea and Valsa sordida were successfully detected as the target pathogens in symptomatic samples by microarray hybridization. The B. dothidea was also found as an endophyte in healthy tissue. [Conclusion] The results indicated that the ArrayTube technique is an effective method for quick detection of poplar canker pathogens, it could be further applied in other forest plant pathogen detection or diagnosis. |
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| Keywords: | canker pathogens Botryosphaeria dothidea microarray hybridization detection ArrayTube |
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