2个MADS-box基因在山葡萄雌花和雄花中的表达分析

为了探明MADS-box基因在山葡萄性别分化中的作用,笔者利用实时荧光定量PCR技术对Vv MADS5和Vv MADS8 2个基因在山葡萄雌、雄花发育过程的表达情况进行分析。结果表明,2个基因在山葡萄雌花和雄花中都有表达,Vv MADS8在雌花和雄花中的表达趋势以及表达量基本相同。Vv MADS5在雌花和雄花中的表达类型相同,而其在雌花中的表达量显著高于雄花。Vv MADS8在花芽膨大期(4月30日)表达量最高,而Vv MADS5在花序展露期(5月14日)表达量最高。Vv MADS8、Vv MADS5参与山葡萄性别分化,但不是控制性别分化的关键基因。

Two MADS-box Genes Expression Analysis in Male and Female Floral Development of Vitis amurensis Rupr.

The paper aims to investigate the role of two MADS-box genes in controlling sex differentiation of Vitis amurensis Rupr.. Real-time PCR was performed to observe the expression profiling of VvMADS5 and VvMADS8 in male and female floral development of Vitis amurensis Rupr. The results indicated that: the two genes were expressed not only in the male floral but also in the female floral; the expression pattern and expression value in the male floral were almost the same as that in the female floral; the expression pattern of VvMADS5 between male and the female was the same, but the expression value in the female was significantly higher than that in the male floral; the expression of VvMADS8 appeared one peak value at bud intumescence stage (April 30), while the highest expression quantity of VvMADS5 appeared at inflorescence show stage (May 14). VvMADS5 and VvMADS8 participated in sex differentiation of Vitis amurensis Rupr., but were not the key genes of controlling sex differentiation.