| 油菜小孢子高效培养体系构建Ⅰ.影响小孢子成胚的若干因素研究 |
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| 引用本文： | 龙卫华,浦惠明,戚存扣,张洁夫,陈松,陈新军,高建芹,陈锋,胡茂龙.油菜小孢子高效培养体系构建Ⅰ.影响小孢子成胚的若干因素研究[J].江苏农业学报,2008,24(6). |
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| 作者姓名： | 龙卫华 浦惠明 戚存扣 张洁夫 陈松 陈新军 高建芹 陈锋 胡茂龙 |
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| 作者单位： | 江苏省农业科学院经济作物研究所,江苏,南京,210014 |
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| 基金项目： | 江苏省科技支撑计划(BE2008369); 国家科技支撑计划(2006BAD01A04) |
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| 摘 要： | 通过对不同田间温度下的油菜花蕾大小、冷处理时间、更换培养液和培养密度等影响因素试验，建立并优化了长江下游生态型甘蓝型油菜高效小孢子培养体系。结果表明，适宜的花蕾大小与田间温度有密切关系，随着温度升高，应适当降低选取花蕾的长度；冷处理对特定的基因型材料的出胚有效，总体影响不大；在高浓度蔗糖的NLN液体培养基培养2d后换NLN13培养液后可使材料的出胚数升高；培养密度以1ml培养液含0.5个花蕾最为合适。
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| 关 键 词： | 油菜 小孢子培养 小孢子成胚 体系构建 |
High Efficiency Microspore Culture System Construction of Brassica Napus Ⅰ.Factors Involved in Microspore Embryogenesis |
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| LONG Wei-hua,PU Hui-ming,QI Cun-kou,ZHANG Jie-fu,CHEN Song,CHEN Xin-jun,GAO Jian-qin,CHEN Feng,HU Mao-long.High Efficiency Microspore Culture System Construction of Brassica Napus Ⅰ.Factors Involved in Microspore Embryogenesis[J].Jiangsu Journal of Agricultural Sciences,2008,24(6). |
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| Authors: | LONG Wei-hua PU Hui-ming QI Cun-kou ZHANG Jie-fu CHEN Song CHEN Xin-jun GAO Jian-qin CHEN Feng HU Mao-long |
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| Abstract: | Four influencing factors were adjusted to yield more embryos,which were bud length under different temperature,cold pretreated time,medium evolution and culture density.The result showed that the correct bud length was very connected with the field temperature,the shorter buds should be chosen as the temperature raised.The cold pretreatment was only effective to some genotype materials,and it did not enhance the embryo number.Changing the medium after incubating with high concentration sucrose NLN liquid fo... |
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| Keywords: | Brassica napus microspore culture microspore embryo system construction |
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