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| 山杏下胚轴再生不定芽影响因子研究(英文) | |
| 引用本文： | 王鸿,马锋旺,王发林,赵秀梅,管清美.山杏下胚轴再生不定芽影响因子研究(英文)[J].果树学报,2005,22(4):399-401,F004. |
| 作者姓名： | 王鸿 马锋旺 王发林 赵秀梅 管清美 |
| 作者单位： | 1. 西北农林科技大学园艺学院,陕西,杨凌,712100;甘肃省农业科学院果树研究所,兰州,730070 2. 西北农林科技大学园艺学院,陕西,杨凌,712100 3. 甘肃省农业科学院果树研究所,兰州,730070 |
| 摘 要： | 山杏成熟种子在改良MS培养基上培养获得下胚轴,将其切段后培养在改良MS培养基+TDZ2.0mg/L+NAA0.5mg/L的再生培养基上,经15d的暗期培养后,其再生频率可达到37%以上。试验表明分裂素TDZ促进不定芽再生的效果优于6-BA,生长素NAA效果优于2,4-D,2-3周龄的下胚轴再生效果较好,但下胚轴的取材部位对再生无显著影响。 |
| 关 键 词： | 杏 下胚轴 再生 |
| 文章编号： | 1009-9980(2005)04-399-03 |
A Study on the factors effecting the adventitious shoot regeneration from hypocotyls of wild apricot |
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| WANG Hong,MA Feng-wang,WANG Fa-lin,ZHAO Xiu-mei,GUAN Qing-mei.A Study on the factors effecting the adventitious shoot regeneration from hypocotyls of wild apricot[J].Journal of Fruit Science,2005,22(4):399-401,F004. | |
| Authors: | WANG Hong MA Feng-wang WANG Fa-lin ZHAO Xiu-mei GUAN Qing-mei |
| Institution: | WANG Hong1,2,MA Feng-wang1*,WANG Fa-lin2,ZHAO Xiu-mei2,GUAN Qing-mei1 |
| Abstract: | Hypocotyls rasied from mature seeds of Prunus armeniaca L var. ansu cultured on modified MS medium were cut into segments and cultured on the regeneration medium, which consisted of modified MS + TDZ 2.0 mg/L+NAA 0.5 mg/L, in dark for 15 d and then exposed to light. The rate of shoot regeneration was higher than 37%. The result showed that TDZ was better than 6-benzylamino-purine (6-BA), and NAA was better than 2,4-D in adventitious shoot regeneration. 2 to 3 week-aged hypocotyls produced better result, but the position effect of explants was not significant for shoot regeneration and callus induction. |
| Keywords: | Apricot Hypocotyl Regeneration |
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