An epidemiological study of Salmonella enterica serovar 4, 12:b:- in broiler chickens in Denmark |
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| Authors: | Chadfield M Skov M Christensen J Madsen M Bisgaard M |
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| Affiliation: | 1. Service de Bactériologie-Hygiene, Assistance Publique–Hôpitaux de Paris (AP-PH), Bicêtre Hospital, Le Kremlin-Bicêtre, France;2. Associated French National Reference Center for Antibiotic Resistance: Carbapenemase-producing Enterobacteriaceae, Le Kremlin-Bicêtre, France;3. EA7361 ‘Structure, dynamic, function and expression of broad spectrum β-lactamases’, Université Paris-Sud, Université Paris-Saclay, LabEx LERMIT, Faculty of Medicine, Le Kremlin-Bicêtre, France;4. Joint Research Unit EERA ‘Evolution and Ecology of Resistance to Antibiotics’, Institut Pasteur, AP-HP, Université Paris Sud, Paris, France;1. Service de Biologie Clinique, Hôpital Foch, Suresnes, France;2. Service de microbiologie et hygiène hospitalière, Hôpital Franco-Musulman Avicenne, Bobigny, France;3. Unité de Bactériologie-Hygiène, Département de Prévention, Diagnostic et Traitement des Infections,Hôpital Henri Mondor, Créteil, France;4. Service de Microbiologie Hygiène Hospitalière, Hôpital Bicêtre, Le Kremlin-Bicêtre, France;5. CNR de la Résistance Aux Antibiotiques, Hôpital Bicêtre, Le Kremlin-Bicêtre, France;1. Department of Pathology and Laboratory Medicine, Nationwide Children''s Hospital;2. Department of Pathology, The Ohio State University, Columbus, OH;3. Department of Pediatrics, The Ohio State University, Columbus, OH |
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| Abstract: | Epidemiological investigations of isolates of Salmonella enterica serovar 4, 12:b:- were carried out to establish particular molecular markers to assign isolates to a common origin. Plasmid profiling demonstrated that over 50% of 291 isolates, obtained between 1991 and 1996, were plasmid-free. The remaining isolates exhibited a common trend in plasmid content of 105 and 2kb. Although no specific correlation to any particular source within the poultry industry was discernible using plasmid analysis, there were indications of clonality with local divergence. Ribotyping with EcoRI demonstrated limited discriminative potential as 96% of the isolates expressed a common profile. Ribotyping with HindIII failed to further differentiate the isolates. IS200 (PstI) typing and PFGE (NotI and XbaI) afforded some degree of further discrimination with selected isolates. Each technique produced four profiles, but dominant profiles were also apparent. Eighteen variables were selected for multivariate logistic regression analysis in order to identify risk areas associated with broiler flocks within the industry. An increased risk for S. 4, 12:b:- infection was only associated with the feedmills used. Random effects at the house and/or farm level were also found to be statistically significant. Of the 16 feedmills associated with the isolation of 4, 12:b:-, six were deemed to be significant risk factors. |
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