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| Prd29A启动子在小麦中的诱导表达活性及大肠杆菌海藻糖合成酶基因的转化 | |
| 引用本文： | 康旭升,刘立科,刘根齐,侯宁,戴秀玉,刘春光. Prd29A启动子在小麦中的诱导表达活性及大肠杆菌海藻糖合成酶基因的转化[J]. 农业生物技术学报, 2007, 15(3): 434-438 |
| 作者姓名： | 康旭升 刘立科 刘根齐 侯宁 戴秀玉 刘春光 |
| 作者单位： | 1. 中国科学院遗传与发育生物学研究所,北京,100101;中国科学院研究生院,北京,100049 2. 中国科学院遗传与发育生物学研究所,北京,100101 3. 中国科学院微生物研究所,北京,100080 |
| 摘 要： | 以gus基因为报告基因，通过瞬时表达测定了Prd29A诱导型启动子在小麦愈伤组织中的诱导表达活性。结果表明，Prd29A-gus基因的表达水平可在不同浓度NaCl盐的胁迫条件下得到显著提高。由此证实，Prd29A启动子在小麦中具有较强的诱导表达活性。在此基础上，将Prd29A诱导型启动子驱动下的大肠杆菌海藻糖合成酶基因（otsA）采用花粉管途径导入目的小麦品系，经PCR筛选、Southern鉴定及转化后代海藻糖含量的测定，获得了一批otsA转基因植株及株系，为进一步选育耐盐抗旱的转基因小麦新品系奠定了基础． |
| 关 键 词： | 小麦 Prd29A启动子 诱导表达 otsA转基因植株 海藻糖 |
| 文章编号： | 1006-1304（2007）03-0434-05 |
| 收稿时间： | 2006-10-08 |
| 修稿时间： | 2006-10-082006-12-07 |
Activity of Inducible Expression of Prd29A Promoter in Wheat and Transformation of Escherichia coli Trehalose-6-phosphate Synthase Gene |
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| KANG Xu-sheng,LIU Li-ke,LIU Gen-qi,HOU Ning,DAI Xiu-yu,LIU Chun-guang. Activity of Inducible Expression of Prd29A Promoter in Wheat and Transformation of Escherichia coli Trehalose-6-phosphate Synthase Gene[J]. Journal of Agricultural Biotechnology, 2007, 15(3): 434-438 | |
| Authors: | KANG Xu-sheng LIU Li-ke LIU Gen-qi HOU Ning DAI Xiu-yu LIU Chun-guang |
| Affiliation: | 1.Insifitute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China; 2.Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China; 3.Graduate University of the Chinese Academy of Sciences, Beijing 100049, China |
| Abstract: | Using the gus gene as reporter gene, inducible expression activity in wheat (Triticum aestivum ) calli of Prd29A promoter was measured by transient expression. The results showed that Prd29A promoter had expression activity under the salt-stress conditions in wheat. Based on the experiment above, Escherichia coli trehalose-6-phosphate synthase gene(otsA) was introduced into NC239 wheat variety through pollen tube pathway, and some otsA transgenic plants were obtained by PCR , Southern blot and trehalose amount analysis. |
| Keywords: | wheat Prd29A promoter inducible expression otsA transgenic plant trehalose |
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