| 粗糙链孢霉杂交试验的新方法 |
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| 引用本文： | 张书芹,蔡明历,刘焰,姚明镜,郝大翠.粗糙链孢霉杂交试验的新方法[J].安徽农业科学,2011,39(13):7588+7591-7588,7591. |
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| 作者姓名： | 张书芹 蔡明历 刘焰 姚明镜 郝大翠 |
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| 作者单位： | 华中农业大学作物学实验教学中心,湖北武汉,430070;华中农业大学作物学实验教学中心,湖北武汉,430070;华中农业大学作物学实验教学中心,湖北武汉,430070;华中农业大学作物学实验教学中心,湖北武汉,430070;华中农业大学作物学实验教学中心,湖北武汉,430070 |
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| 基金项目： | 湖北省教改项目(20060157) |
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| 摘 要： | 目的]改进粗糙链孢霉杂交试验的方法。方法]对粗糙链孢霉培养条件、杂交方法、最佳取材时间及子囊孢子成熟时对应的子囊果的形状进行研究。结果]粗糙链孢霉菌种的活化、杂交均可在玉米培养基上培养;在杂交时,将菌丝挑到无菌水中制成菌液,用菌液接种,方便快捷;在25℃,21 d左右取黑色长刺状凸起的子囊果成熟度好,压片效果好。结论]试验结果可应用于遗传学教学实践。
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| 关 键 词： | 粗糙链孢霉 培养条件 形状 教学实践 |
New Methods of the Cross Experiment of Neurospore crassa |
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| ZHANG Shu-qin et al.New Methods of the Cross Experiment of Neurospore crassa[J].Journal of Anhui Agricultural Sciences,2011,39(13):7588+7591-7588,7591. |
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| Authors: | ZHANG Shu-qin |
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| Institution: | ZHANG Shu-qin et al(The Experiment Teaching Center of Crop Science,Huazhong Agricultural University,Wuhan,Hubei 430070) |
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| Abstract: | Objective] The aim of the study was to modify the cross experiment of Neurospore crassa.Method] Cultivation conditions and the proper shape of the ascocarp,especially some detailed experiment steps were studied.Result]The study showed that in the corn culture medium,the activation and the cross experiment of Neurospore crassa grew well;at the time of cross experiment,bacteria liquid were prepared by using hypha in sterile water,the inoculation of bacteria liquid was convenient and quick.After cultivating... |
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| Keywords: | Neurospore crassa Cultivation conditions Experimental teaching |
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