| 菌株I13产碱性蛋白酶的酶学性质研究 |
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| 引用本文： | 顾艳丽,刘赛男,张慧,李宪臻.菌株I13产碱性蛋白酶的酶学性质研究[J].安徽农业科学,2011(13):7574-7575. |
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| 作者姓名： | 顾艳丽 刘赛男 张慧 李宪臻 |
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| 作者单位： | 大连工业大学生物工程学院; |
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| 基金项目： | 国家自然科学基金项目(30670067) |
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| 摘 要： | 目的]研究菌株I13产碱性蛋白酶的酶学性质。方法]从一株碱性蛋白酶生产菌株I13中得到了碱性蛋白酶粗酶液,并研究了温度和pH对该酶液酶活力的影响。结果]所产碱性蛋白酶的最适反应温度为40℃,在20~30℃范围内仍保持了最大活力的40%以上;最适反应pH为10.5,在pH 8.0~11.0范围内保持了最大活性的90%以上,有较宽的pH谱。结论]该碱性蛋白酶具有开发成为洗衣粉添加剂的巨大潜力。
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| 关 键 词： | 碱性蛋白酶 筛菌 酶学性质 |
Properties of Alkaline Protease Produced by Strain I13 |
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| GU Yan-li et al.Properties of Alkaline Protease Produced by Strain I13[J].Journal of Anhui Agricultural Sciences,2011(13):7574-7575. |
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| Authors: | GU Yan-li |
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| Institution: | GU Yan-li et al(School of Bioengineering,Dalian Polytechnic University,Dalian,Liaoning 116034) |
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| Abstract: | Objective] This study was aimed to investigating properties of alkaline protease produced by strain I13.Method] Crude enzyme of alkaline protease was obtained from alkaline protease produced by strain I13,while effects of temperature and pH value on enzyme activity was also investigated in this study.Result] The optimal temperature of alkaline protease produced by strain I13 was 40 ℃,and over 40% of the largest enzyme activity still maintained within the range from 20 to 30 ℃.The optimal pH value was 10.5,and over 90% of the largest enzyme activity still maintained within the range from 8.0 to 11.0,which had broader pH value spectrum.Conclusion] This alkaline protease has huge potential to be developed into washing-powder additive. |
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| Keywords: | Alkaline protease Screening Enzymatic characterization |
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