| 黑麦特异性PCR反应体系的建立 |
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| 引用本文： | 任如意.黑麦特异性PCR反应体系的建立[J].安徽农业科学,2011,39(13):7582-7584,7946. |
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| 作者姓名： | 任如意 |
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| 作者单位： | 牡丹江师范学院生命科学与技术学院,黑龙江牡丹江,157012 |
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| 基金项目： | 黑龙江省普通高校青年学术骨干计划项目(1152G047); 牡丹江师范学院大学生创新项目 |
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| 摘 要： | 目的]建立黑麦特异性PCR反应优化体系。方法]以普通小麦＂中国春＂、S165、黑麦、八倍体小黑麦、六倍体小黑麦为试验材料,研究了模板DNA、引物、dNTPs、Mg2＋浓度、TaqDNA聚合酶用量及退火温度对黑麦特异性PCR反应体系的影响。结果]采用改良的CTAB DNA微量提取法可以得到高质量的基因组DNA,满足PCR反应模板的要求,黑麦特异PCR扩增反应体系为：在25μl反应体系中,10×缓冲液,1.5 mmol/LMgCl2,200μmol/LdNTP,40 ng引物,40~60 ng模板DNA,1 UTaq酶。结论]建立了适宜的黑麦特异PCR扩增反应体系,为小麦背景下黑麦外源种质的检测奠定了基础。
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| 关 键 词： | 黑麦 PCR反应体系 优化 |
Establishment and Optimization of Rye-specific PCR Reaction System |
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| REN Ru-yi.Establishment and Optimization of Rye-specific PCR Reaction System[J].Journal of Anhui Agricultural Sciences,2011,39(13):7582-7584,7946. |
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| Authors: | REN Ru-yi |
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| Institution: | REN Ru-yi(College of Life Science and Technology,Mudanjiang Normal University,Mudanjiang,Heilongjiang 157012) |
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| Abstract: | Objective] The aim was to establish the optimal rye-specific PCR reaction system for rye.Method] The ordinary wheat Chinese Spring,S165,rye,octoploid triticale and hexaploid triticale were used as materials to carry out study on the effect of the amount of template DNA,primers,dNTPs,Mg2+ concentrations,Taq DNA polymerase and annealing temperature on the rye-specific PCR reaction system of rye.Result] The genomic DNA extracted by modified CTAB DNA extraction method showed high quality,which was satisfied ... |
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| Keywords: | Rye PCR reaction system Optimization |
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