硒对体外培养小鼠成骨细胞形态、增殖和活力的影响

本文旨在探讨不同浓度亚硒酸钠溶液对体外培养的小鼠成骨细胞的形态、增殖及活力的影响。以出生24h的昆明小鼠颅骨为材料,通过酶消化法来分离成骨细胞,并分别接种于不同浓度的亚硒酸钠培养液(0.00、0.05、0.1、0.2、0.4、0.6、0.75、1.0 mg/L)中,倒置显微镜下观察成骨细胞的形态变化,采用MTT法测定细胞增殖率,结晶紫染色法测定细胞活力。48 h内,浓度高于0.4 mg/L时亚硒酸钠抑制细胞增殖且降低细胞活力,而低于0.2mg/L时亚硒酸钠可促进细胞增殖,增强了细胞活力。结果显示硒对成骨细胞的增殖和活力呈双向调节,硒作用呈浓度剂量依赖性。

Effects of Sodium Selenite on Morphology, Proliferation and Viability of Mice Osteoblast Cultured in vitro

To observe the effect of the selenium with different concentrations on the morphology,proliferation and viability of mice osteoblast in vitro.The 24 hour-old mice osteoblasts,which were obtained from skull with complete enzyme digestion,were cultured in DMEM solution seperatively containing various concentrations of sodium selenite(0.00,0.05,0.1,0.2,0.4,0.6,0.75,1.0 mg/L).The morphological changes were viewed;The proliferation rate and cell viability were measured by MTT and crystal violet staining,respectively.The results indicated that the proliferative capacity and viability of osteoblasts exposed to Na2SeO3,was increased at the concentration of 0.2 mg/L in 48 h,however they were inhibited over 0.4 mg/L Na2SeO3.Selenium has two-side effects for proliferation and viability of osteoblasts.It is time-dossage dependent.