| 高频率生菜植株再生及转化体系的建立 |
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| 引用本文： | 刘凡 李岩S.高频率生菜植株再生及转化体系的建立[J].华北农学报,1996,11(1):109-113. |
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| 作者姓名： | 刘凡 李岩S |
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| 作者单位： | 北京市农林科学院蔬菜研究中心 |
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| 摘 要： | 通过对两种生菜及其不同外植体在培养基上不定芽发生能力的筛选，得到“大湖３６６”叶切片不定芽发生率高达８７％的培养体系，并且丛生状不定芽可陆续发生。将叶切片与农杆菌共培养，对经ｋｍ筛选后所得再生株进行Ｘ－Ｇｌｕ染色，兰色反应阳性率为５０％。
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| 关 键 词： | 生菜 遗传转化 农杆菌 植株再生 |
High Efficiency Regeneration and Transformation of Lettuce (Lactuca sativa var. capitata L.)Using Agrobacterium Vector |
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| Liu Fan, Li Yan, Cao Mingqing.High Efficiency Regeneration and Transformation of Lettuce (Lactuca sativa var. capitata L.)Using Agrobacterium Vector[J].Acta Agriculturae Boreali-Sinica,1996,11(1):109-113. |
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| Authors: | Liu Fan Li Yan Cao Mingqing |
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| Abstract: | An efficient procedure for obtaining transgenic lettuce plants using agrobacterium vecis described. The young leaf sections,when cultured on MS medium with 0. 5mg/L BA,0. 1 mg/L NAA,had a 87% plant regeneration rate,and more than one shoot were regenerated per regenerable explant during culturing. After co-cultured with ugrobacteria,the explants had a 65 % regeneration rate on MS medium with 100mg/L kanamycin. These regenerated plants showed a 50% positive reaction frequency when their leaf slices incubated with X-Glu solution. |
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| Keywords: | Lettuce Regeneration Transformation Agrobacterium |
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