| 珍稀濒危植物珙桐初代培养研究 |
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| 引用本文： | 植爽,胡艳,曹婧,肖小君,陈文年.珍稀濒危植物珙桐初代培养研究[J].江苏林业科技,2014(4):29-32. |
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| 作者姓名： | 植爽 胡艳 曹婧 肖小君 陈文年 |
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| 作者单位： | 内江师范学院生命科学学院;四川省高等学校特色农业资源研究与利用重点实验室; |
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| 基金项目： | 国家级大学生创新创业训练计划项目(201310640010);内江师范学院校级重点项目(12NJZ04);内江市科技支撑计划项目(12109);内江师范学院大学生科研项目(13NSD-76) |
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| 摘 要： | 以珙桐芽体为试材,采用WPM、1/2 MS、B5三种基本培养基,附加6-BA、NAA、GA34因素3水平正交实验设计,同时研究了不同外植体消毒时间、剥离方式、芽体长度对珙桐初代培养的影响。结果表明:用75%酒精30 s+0.1%HgCl220 min消毒效果较好,外植体长度大于0.5 cm,剥离芽体至剩余鳞片叶1~2个的处理方式有利于降低污染率,最佳芽诱导的培养基配方为:WPM+6-BA 1.0 mg/L+NAA 0.05 mg/L+AC 0.5 g/L。
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| 关 键 词： | 珙桐 诱导 外植体 初代培养 |
Initial culture study of endangered plant Davidia involucrata Baill. in vitro micropropagation |
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| Institution: | ZHI Shuang, HU Yan , CAO Jing , XIAO Xiao-jun , CHEN Wen-nian ( 1. School of Life Sciences, Neijiang Normal University, Neijiang 641112, China; 2. Key Laboratory of Colleges and Universities in Sichuan for Research and Utilization of Distinctive Agricuhural Undertakings, Neijiang 641112, China) |
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| Abstract: | Using basal medium of WPM,1/2MS and B5 which added 6-BA,NAA and GA3,the time of different explant disinfection,the way of stripping and the length of buds of Davidia involucrata were studied. The results showed that the disinfection effect was appropriate by adopting 75% alcohol for 30 and 0. 1% HgCl2 for 20 min,it was good for reducing pollution to us the explants which were more than 0. 5 cm and the stripping way to the rest 1 to 2 scale leaves. And the best medium for buds induction was WPM ＋ 6-BA 1. 0 mg/L ＋ NAA 0. 05 mg/L ＋ AC 0. 5 g/L. |
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| Keywords: | Davidia involucrata Baill Induction Explant Initial culture |
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