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工业化大豆蛋白7S和11S组分分离技术研究进展
引用本文:徐婧婷,任建华,叶凌凤,郭顺堂. 工业化大豆蛋白7S和11S组分分离技术研究进展[J]. 大豆科学, 2010, 29(3)
作者姓名:徐婧婷  任建华  叶凌凤  郭顺堂
作者单位:中国农业大学,食品科学与营养工程学院,北京,100083;中国农业大学,食品科学与营养工程学院,北京,100083;中国农业大学,食品科学与营养工程学院,北京,100083;中国农业大学,食品科学与营养工程学院,北京,100083
摘    要:该文围绕目前工业化生产11S和7S组分的分离技术成果,结合实验室的分离技术(Guo法),比较了各种分离方法的差异.结果表明:分离提取技术利用的原理几乎均建立在"碱溶酸提"和"冷沉"作用基础之上.Wu首次成功地进行了7S和11S的工业化分离,之后的研究多是对此方法的改进和修饰.而Guo法是唯一采用中性条件抽提的工业化分离方法,在节省了原料碱的同时,避免了蛋白的变性.同时,该文提出了目前工业化生产大豆组分蛋白中存在的问题,虽然不断在加工方式上进行改进,但仍存在步骤复杂、产率低、生产成本高等方面的问题,因此作者进一步对实现工业化分离大豆组分蛋白提出了需要改进和努力的方向.

关 键 词:大豆蛋白  分离技术  工业化生产  7S富集蛋白  11S富集蛋白  产量  纯度

Technical Research of Industrialized 7S and 11S Soy Protein Fractionation
XU Jing-ting,REN Jian-hua,YE Ling-feng,GUO Shun-tang. Technical Research of Industrialized 7S and 11S Soy Protein Fractionation[J]. Soybean Science, 2010, 29(3)
Authors:XU Jing-ting  REN Jian-hua  YE Ling-feng  GUO Shun-tang
Abstract:Combining with our isolation techniques (method of Guo), this article compared different separation methods of 11S and 7S fractions with industrial methods. The principles of most separation techniques are "alkali extraction and acid precipitation" and "cold precipitation" effects. Wu's method succeeded in isolation 11S and 7S fraction for the first time, the later researches mostly focus on its improvement. The method of Guo is the only method to extract protein in a neutral condition, thus saved the usage of alkali and avoided the denaturation of protein. Meanwhile, this article pointed out the problems in the current industrial production. Although lots of improvements were made on the separation process, problem still exist, such as complicated procedures, low yields, high costs of production and so on. Therefore, the writer further proposed the directions of efforts and improvement for the realization of industrial production.
Keywords:Soy protein  Separation techniques  Industrial production  7S rich fraction  11S rich fraction  Yield  Purity
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