| 生长调节剂和外植体对油菜离体培养愈伤诱导的影响 |
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| 引用本文： | 田志宏,杨成军.生长调节剂和外植体对油菜离体培养愈伤诱导的影响[J].湖北农业科学,2003(4):41-43. |
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| 作者姓名： | 田志宏 杨成军 |
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| 作者单位： | 湖北农学院生命科学学院,湖北,荆州,434025 |
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| 基金项目： | 湖北农学院科研基金重点项目资助(HNZD2 0 0 0 0 1) |
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| 摘 要： | 以双低优质油菜品种中双 7号的无菌苗为材料 ,探索了以子叶、下胚轴和幼根为外植体 ,及不同生长调节剂组合对愈伤诱导的影响作用。结果表明 ,生长调节剂是愈伤诱导成功的关键因子 ,2 ,4-D是诱导愈伤所必需的 ,以MS培养基含 1mg·L- 1 2 ,4-D与 2mg·L- 1 6 -BA配比为佳 ;同时子叶优于下胚轴和幼根作为外植体进行愈伤诱导 ,可获得出愈率高、增殖快、质量好的愈伤组织
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| 关 键 词： | 油菜 离体培养 愈伤组织 生长调节剂 外植体 |
| 文章编号： | 0439-8114(2003)04-0041-03 |
| 修稿时间： | 2003年5月6日 |
Effect of growth regulator and explants on callus induction in vitro culture of rape |
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| TIAN Zhi-hong,YANG Cheng-jun.Effect of growth regulator and explants on callus induction in vitro culture of rape[J].Hubei Agricultural Sciences,2003(4):41-43. |
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| Authors: | TIAN Zhi-hong YANG Cheng-jun |
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| Abstract: | The effects of different growth regulator combinations and explants (cotyledon, hypocotyl and young root) upon callus induction of rape were studied, based on aseptically seedlings of "Zhongshuang No. 7", a double low elite variety The results indicated that growth regulator was crucial to induce callus of rape, in especial 2,4-D, the optimal induction medium which could induce callus was MS medium supplemented with 1 mg/L 2,4-D and 2 mg/L 6-BA, and as explants, cotyledon was fit for callus induction than hypocotyl and young root, to induce callus with high induction rate, quick growth and good quality |
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| Keywords: | rape in vitro culture callus growth regulator explants |
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