| 影响大豆疫霉菌(Phytophthora sojae)游动孢子产生的条件 |
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| 引用本文： | 左豫虎,臧忠婧,刘惕若. 影响大豆疫霉菌(Phytophthora sojae)游动孢子产生的条件[J]. 植物病理学报, 2001, 31(3): 241-245 |
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| 作者姓名： | 左豫虎 臧忠婧 刘惕若 |
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| 作者单位： | 黑龙江八一农垦大学植物免疫研究室, 密山市 158308 |
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| 基金项目： | 国家"九五”科技攻关项目(G95-01-05-03);黑龙江省科委"九五”重大科技攻关项目(G97B2-3) |
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| 摘 要： | 间歇性地给菌丝块换水可以诱导菌丝产生孢子囊,进而释放游动孢子。挑取5~8块直径为8mm于利马豆或V8汁平板上培养4~8d的菌块,置于直径7cm的培养皿内,加入蒸馏水正好没过菌块表面,每30 min换水一次,换水4次后加入15 ml Petri培养液,25℃、黑暗条件下培养18~20 h,可诱发菌块大量产孢。
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| 关 键 词： | 大豆疫霉菌 游动孢子 产生条件 |
| 文章编号： | 0412-0914(2001)03-0241-05 |
| 修稿时间： | 2000-04-26 |
STUDIES ON PRODUCTION CONDITION OF ZOOSPORES OF Phytophthora sojae |
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| ZUO Yu hu,ZANG Zhong jing,LIU Ti ruo. STUDIES ON PRODUCTION CONDITION OF ZOOSPORES OF Phytophthora sojae[J]. Acta Phytopathologica Sinica, 2001, 31(3): 241-245 |
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| Authors: | ZUO Yu hu ZANG Zhong jing LIU Ti ruo |
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| Affiliation: | Research Laboratory of Plant Immunology, Helongjiang August First Land Reclamation University, Mishan 158308, China |
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| Abstract: | By changing water intermittently, we can induce mycelia of Phytophthora sojae to produce sporangia that release zoospores. The isolates of P.sojae were cultured on LBA or V8A for 4 8 da ys. Five or more culture blocks (8 mm diameter) were cut and transferred to a 7 cm plate with distilled water just covering the surface of culture blocks. 15 ml Petri nutrient solution was added into the culture dish after having changed water four times at 30 mi nutes interval. After incubation under 25℃ in darkness for 18 20 hours, mycelia could produce a large number of sporangia that release zoospores. |
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| Keywords: | Phytophthora sojae zoospores production condition |
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