大豆凝集素(SBA)含量遗传分析与QTL定位

大豆凝集素(Soybean agglutinin,SBA)为大豆中含量较高、作用较强主要抗营养因子之一,影响大豆食品和饲料安全,降低或去除大豆籽粒和产品中凝集素成为大豆食品和饲料工业亟待解决问题。文章以合丰45号(高SBA含量)×太平川黑豆(低SBA含量)杂交组合及衍生201个稳定株系组成的F78重组自交系(RIL)群体为试验材料,在3年(2011~2013)1个样点(哈尔滨)种植环境下对大豆凝集素含量作遗传模型和QTL分析。结果表明,SBA含量符合2对主基因+多基因混合遗传模型;利用134对多态性SSR引物扩增RIL群体,构建遗传图谱,对SBA含量相关作QTL分析。共检测到4个与SBA含量相关QTL,每个QTL均重复检出2次,稳定性较好,其中SbaHTC1-1(Satt139~Satt578)和SbaHTD1b-1(Satt537~Satt189)2个QTL位点两年检测结果加性效应值均达显著水平,且遗传贡献率较高,为主效QTL。利用分子标记遗传图谱,定位与SBA含量相关QTL,为改良大豆凝集素含量提供理论依据和技术支持。

Genetic and QTL analysis of Soybean agglutinin (SBA) content

Soybean agglutinin (SBA)was one of the antinutritional factors with stronger function and higher content in soybean,that could be an serious impact on the safety of the soybean food and feed.Reducing or eliminating SBA in soybean seed and products had become important on soybean food and feed industry.In order to map steady and repeatable QTLs of SBA content,a F7:8 RIL population containing 201 lines derived from cross between Hefeng 45 as female and Taipingchuan blacksoy as male parent were used in this experiment.Analysis of QTL and genetic model on SBA content was carried out in different environments.The results of separation analysis showed that SBA content accord with the 2 major genes+more gene hybrid genetic model.134 pairs of SSR primers had been used to analysis in RIL population,further construction of genetic map and QTL analysis for SBA content were conducted.The result of QTL analysis showed that 4 QTL were detected associated with SBA content in different environments.Each QTL were detected stability in different environment.Two QTL of SbaHTD1b-1 and SbaHTC1-1 reached significant level and explained higher phenotypic variation in different environments.These QTL might provide the valuable information for soybean molecular marker assistant breeding selection.