楤木激素自养型细胞系生物学特性

以前期研究获得的楤木激素自养型细胞系为研究材料,利用分光光度和高压液相色谱(HPLC)法分析了该细胞系,即生长量,3种抗氧化酶(超氧化物歧化酶SOD、过氧化物酶POD、过氧化氢酶CAT)活性,细胞壁(醇不容提取物)质量分数,细胞壁中纤维素、半纤维和总果胶的质量分数,以及3种植物内源激素(植物生长素IAA、赤霉素GA3、脱落酸ABA)的生物化特性。结果显示:激素自养型细胞系高速生长,在培养的第30天达到最大生长量,鲜质量和干质量分别是非激素自养型细胞系的2.64、2.22倍。在0~30 d,激素自养型细胞系中3种抗氧化酶活性都显著高于非激素自养型细胞系。激素自养型细胞系中的细胞壁质量分数(8.94 mg·g~(-1))明显低于培养苗中的(13.15 mg·g~(-1)),高于非激素自养型细胞系的;细胞壁中总果胶的质量分数显著低于非激素自养型细胞系和组培苗的;纤维素质量分数与非激素自养型细胞系的差异不显著;半纤维素质量分数显著高于非激素自养型细胞系的。非激素自养型细胞系中GA3质量分数是激素自养型细胞系的2.6倍,两种细胞系中ABA质量分数差异不显著,激素自养型细胞系中IAA质量分数与非激素自养型细胞系中的无显著差异。

Biological Characteristics of the Habituated Cell Line of Aralia elata Seem

By spectrophotometer and HPLC, we studied the biological characteristics of the habituated cell line of Aralia elata Seem including the growth, antioxidant enzyme activities (peroxidase POD, superoxide dismutase SOD and catalase CAT), cell wall composition analysis (pectin, fibrin and half-fibrin) and endogenous phytohormones (IAA, ABA, GA3) with the inhabituated cell line and the plant in vitro of A.elata Seem as controls.The habituated cell line more quickly grew than that of inhabituated during the culture of 30d, and the fresh and dry weights of the habituated cell were 2.64 and 2.22 times than those of inhabituated one, respectively.The activities of three antioxidant enzymes in habituated cell line were obviously higher than those in inhabituated one.The cell wall content of habituated cell line was much lower than that of the plant, and higher than that of inhabituated cell line.In the cell wall, the contents of pectin and fibrin in habituated cell line were lower significantly than those in plant and in inhabituated cell one;its fibrin content wasn''t different significantly with that in inhabituated one, and its half-fibrin content was significantly higher than that in habituated one.The content of GA3 in inhabituated cell line was 4.35 times than that in habituated one, and there was almost no difference of the content of ABA in habituated and inhabituated cell line.There was no significant difference in the content of IAA between in the habituated cell line and the inhabituated one.