低能氮离子束诱变选育捕食线虫性真菌——少孢节丛孢菌

为获取理想的生物防治捕食线虫性真菌菌株,达到利用自然天敌有效控制家畜寄生性线虫病的目的,作者利用低能氮离子注入诱变捕食线虫性真菌——少孢节丛胞菌(Arthrobotrys oligospora)的分生孢子,测定分生孢子萌发后菌丝的生长速度、产孢能力及菌株对线虫幼虫的捕食率,筛选正突变株。结果表明,低能氮离子束具有诱变少孢节丛孢菌的潜能,诱变后的1株少孢节丛孢菌的生长速度、产孢量和对线虫幼虫的捕食率呈现出了正突变效果。

Study on Mutation Breeding of Arthrobotrys oligospora by Low Energy N+ Implantation

The objective of this study was to get effective strain of nematode-trapping fungus-Arthrobotrys oligospora,by which parasitic nematodes of livestocks could be controlled and chemical drugs may be replaced partly or completely.First of all,the mutagenic effect of the low-energy nitrogen ion beam on the original strain-A.oligospora were studied,then the survival rate of strains injected by different doses of nitrogen ion were determined to select the best injection parameter: energy,dose.Later,the strains were screened out according to mycelial growth rate,sporulation,the trapping rate and resistance to the digestion of the sheep.The results showed that ion beam implantation could be an efficient way to improve the characters of nematode-trapping fungus,such as growth rate,sporulation quantity and nematode-trapping rate,and it was the first time to use ion beam in mutation breeding of nematode-trapping fungi.