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| 猪蛋白酪氨酸磷酸酶-1B基因第7内含子多态性与猪脂肪沉积性状的关联性 | |
| 引用本文： | 王丽霞,魏金涛,冉志平,钱运国,喻婷,熊远著.猪蛋白酪氨酸磷酸酶-1B基因第7内含子多态性与猪脂肪沉积性状的关联性[J].畜牧兽医学报,2012,43(7):1015-1019. |
| 作者姓名： | 王丽霞 魏金涛 冉志平 钱运国 喻婷 熊远著 |
| 作者单位： | 1. 华中农业大学农业部猪遗传育种重点开放实验室,武汉430070;武汉市农业科学技术研究院畜牧兽医科研所,武汉430065 2. 湖北省农业科学院畜牧兽医研究所,武汉,430064 3. 武汉市农业科学技术研究院畜牧兽医科研所,武汉,430065 4. 华中农业大学农业部猪遗传育种重点开放实验室,武汉,430070 |
| 基金项目： | "863"国家高技术研究发展计划，"973"国家重大基础研究项目 |
| 摘 要： | 本研究旨在分析蛋白酪氨酸磷酸酶-1B(PTP-1B)基因部分SNP与猪脂肪沉积性状的关联性。选取国外瘦肉型猪种大约克夏16头、长白猪21头和中国脂肪型地方猪种梅山猪22头以及"大白×梅山"F2代资源家系213头为研究材料,利用PCR-Eco88I-RFLP方法检测PTP-1B基因第7内含子序列存在G/A突变的多态性,并进行方差分析。结果表明,大白和长白猪种为单一的A等位基因,而梅山猪种以杂合子GA基因型为主,对213头"大白×梅山"F2代资源家系进行性状关联分析,该位点与猪板油质量、内脂率、胸腰椎间背膘厚等脂肪沉积性状显著相关(P<0.05),且杂合子GA基因型个体的板油质量和内酯率都最低(GA基因型个体 |
| 关 键 词： | 蛋白酪氨酸磷酸酶-1B 基因 猪 脂肪沉积 多态性 |
Association of Polymorphisms of Porcine Protein Tyrosine Phosphatese 1B Gene Intron 7 with Fat Deposition Traits |
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| WANG Li-xia , WEI Jin-tao , RAN Zhi-ping , QIAN Yun-guo , YU Ting , XIONG Yuan-zhu.Association of Polymorphisms of Porcine Protein Tyrosine Phosphatese 1B Gene Intron 7 with Fat Deposition Traits[J].Acta Veterinaria et Zootechnica Sinica,2012,43(7):1015-1019. | |
| Authors: | WANG Li-xia WEI Jin-tao RAN Zhi-ping QIAN Yun-guo YU Ting XIONG Yuan-zhu |
| Institution: | 1(1.Key Laboratory of Pig Genetics and Breeding of Ministry of Agriculture,Huazhong Agricultural University,Wuhan 430070,China;2.Institute of Animal and Veterinary Science,Wuhan Academy of Agricultural Science and Technology,Wuhan 430065,China;3.Institute of Animal Husbandry and Veterinary,Hubei Academy of Agriculture Science,Wuhan 430064,China) |
| Abstract: | To discuss the association of polymorphism of porcine protein tyrosine phosphatese 1B gene with fat deposition traits,in this study,the sequence of intron 7 of the porcine PTP-1B gene was obtained from Large White,Landrace and Meishan pigs.Sequence comparison revealed a G/A mutation which was detected by PCR-Eco88 I-RFLP.The allele distribution revealed that Large White and Landrace pigs had only A allele,and GA was preponderant genotype in Meishan pigs.Association analysis between the polymorphism of PTP-1B gene and fat deposition traits was performed in 213 Large White × Meishan F2 pigs.Significant associations of the genotypes with fat deposition traits such as leaf fat weight(LFW),internal fat rate(IFR) and thorax-waist fat thickness were found.The individuals with GA genotype had lowest leaf fat weight and internal fat rate than the individuals with the other genotypes(GA |
| Keywords: | protein tyrosine phosphatese 1B gene porcine fat deposition polymorphism |
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