山羊瘤胃产甲烷古菌多样性及与其他动物瘤胃的比较

旨在研究山羊瘤胃产甲烷古菌的多样性并与其他动物瘤胃进行比较。采用产甲烷菌特异性引物Met86F/Met1340R研究山羊瘤胃产甲烷古菌16SrRNA基因的多样性,随机挑选100个克隆进行序列分析。结果表明,所有克隆经限制性内切酶酶切后共获得16个OTU,绝大多数为甲烷短杆菌,其中与甲烷短杆菌菌株AK-87、ZA-10、OCP、SM9和30Y序列最相近的克隆数分别为58%、19%、7%、2%和2%,还有与Methanosphaera stadtmanae(1%)、Methanobacterium aarhusense(2%)、Aciduliprofundum boonei(3%)、Methanobrevibacter sp.AK-87(4%)和Methanobrevibactersp.1Y(2%)等相似的古菌序列。将研究结果与已经报道的关于牛和绵羊瘤胃产甲烷菌多样性比较,发现不同PCR引物可检测出不同菌群结构,而饲料类型、动物种类可影响瘤胃产甲烷菌的菌群结构。

Molecular Diversity of Rumen Methanogens from the Goat and Its Comparison with Other Ruminants

This experiment was conducted to study the molecular diversity of rumen methanogens from goat and its comparison with other ruminant.The methanogen-specific primers Met86F/Met1340R was used to analyze the 16S rRNA gene diversity of methanogens.One hundred clones were randomly picked up for sequence analysis.The result showed that sixteen OTUs were obtained after RFLP analysis,and the majority belonged to the Methanobrevibacter strains,AK-87(58%),and others to ZA-10(19%),OCP(7%),SM9(2%),and 30Y(2%),and Methanosphaera stadtmanae-like(1%),Methanobacterium aarhusense-like(2%),Aciduliprofundum boonei-like(3%),Methanobrevibacter sp.AK-87-like(4%) and Methanobrevibacter sp.1Y-like(2%) sequences,respectively.With comparison to rumen methanogens from other ruminants reported in previous studies,it can be concluded that the structure of methanogenic communities in the rumen can be affected by the different primers,feed types and animal species.