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| SLA-DQA基因在大约克、苏太和梅山猪断奶仔猪中组织表达差异分析 | |
| 引用本文： | 訾臣,吴正常,刘璐,杜子栋,黄小国,杨建生,朱国强,吴圣龙,包文斌.SLA-DQA基因在大约克、苏太和梅山猪断奶仔猪中组织表达差异分析[J].畜牧兽医学报,2012,43(5):832-837. |
| 作者姓名： | 訾臣 吴正常 刘璐 杜子栋 黄小国 杨建生 朱国强 吴圣龙 包文斌 |
| 作者单位： | 1. 扬州大学动物科学与技术学院江苏省动物遗传繁育与分子设计重点实验室,扬州,225009 2. 扬州大学兽医学院,扬州,225009 3. 江苏省现代种猪分子选育工程技术研究中心,常州213149;江苏(扬州)规模猪场高效健康养殖公共技术服务中心,扬州225009 4. 江苏(扬州)规模猪场高效健康养殖公共技术服务中心,扬州,225009 5. 扬州大学兽医学院,扬州225009;江苏省现代种猪分子选育工程技术研究中心,常州213149;江苏(扬州)规模猪场高效健康养殖公共技术服务中心,扬州225009 6. 扬州大学动物科学与技术学院江苏省动物遗传繁育与分子设计重点实验室,扬州225009;江苏省现代种猪分子选育工程技术研究中心,常州213149;江苏(扬州)规模猪场高效健康养殖公共技术服务中心,扬州225009 |
| 基金项目： | 转基因生物新品种培育科技重大专项，江苏省高校优势学科建设工程资助项目，苏州市科技支撑计划，常州市科技攻关项目 |
| 摘 要： | 本研究旨在通过对SLA-DQA基因在苏太猪大肠杆菌F18菌株抗性群体和大约克猪、梅山猪断奶仔猪各组织间的表达规律及其在3个群体间表达差异的分析,探讨SLA-DQA基因与国内外猪品种断奶仔猪不同水平免疫机能和大肠杆菌抗性间的潜在关系。研究结果显示,SLA-DQA基因在3个猪群体所检测的11个组织中均表达,并且表达规律基本一致,在肺脏、脾脏、淋巴结等免疫器官中的表达量较高,在胃、十二指肠、空肠等消化道中表达量居中。SLA-DQA基因在苏太猪所有检测组织中的表达量均最高,大约克中次之,梅山猪中表达量最低。尤其在肺脏、淋巴结和胸腺3个组织中,SLA-DQA基因在苏太猪中的表达量均显著高于其在梅山猪中的表达量;在胸腺组织中,SLA-DQA基因在苏太猪中的表达量显著高于大约克猪。研究结果表明,SA-DQA基因在断奶仔猪受大肠杆菌F18菌株病原菌侵袭后引起的一系列生理变化和应答过程中发挥着重要的抗原递呈和激发机体免疫的作用。 |
| 关 键 词： | 猪 SLA-DQA基因 免疫应答 F18大肠杆菌 |
Tissue Expression Differentiation of SLA-DQA Gene in Yorkshire,Sutai and Meishan |
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| ZI Chen , WU Zheng-chang , LIU Lu , DU Zi-dong , HUANG Xiao-guo , YANG Jian-sheng , ZHU Guo-qiang , WU Sheng-long , BAO Wen-bin.Tissue Expression Differentiation of SLA-DQA Gene in Yorkshire,Sutai and Meishan[J].Acta Veterinaria et Zootechnica Sinica,2012,43(5):832-837. | |
| Authors: | ZI Chen WU Zheng-chang LIU Lu DU Zi-dong HUANG Xiao-guo YANG Jian-sheng ZHU Guo-qiang WU Sheng-long BAO Wen-bin |
| Institution: | 1,3,4 (1.Key Laboratory for Animal Genetics,Breeding,Reproduction and Molecular Design of Jiangsu Province,College of Animal Science and Technology,Yangzhou University,Yangzhou 225009, China;2.College of Veterinary Medicine,Yangzhou University,Yangzhou 225009,China; 3.Jiangsu Engineering Research Centre for Molecular Breeding of Breeder Pig,Changzhou 213149,China;4.Jiangsu(Yangzhou) Public Technical Service Centre for Efficient and Healthy Culture Technology in Large-scale Swine Farms,Yangzhou 225009,China) |
| Abstract: | The aim of this study was to analyse SLA-DQA gene expression in post-weaning piglets of Yorkshire,Meishan and E.coli F18-resistant resource population of Sutai by real-time PCR and to investigate the potential relationship between SLA-DQA gene and the different immune capacity of Chinese and exotic pig breeds,as well as the resistance to E.coli F18.SLA-DQA gene expression was detected in 11 tissues and the expression pattern was consitent in the three pig breeds,the expression level was the higher in lung,spleen and lymph node,the moderate in stomach,duodenum and jejunum.Meanwhile,the expression level of SLA-DQA gene was the highest in all tissues detected in Sutai piglets,followed by Yorkshire and Meishan.The expression level in lung,lymph node and thymus in Sutai was significantly higher than that in Meishan.In addition,there was significant difference in SLA-DQA gene’s expression between Sutai and Meishan pigs.From above analysis,it was concluded that SLA-DQA gene has important function of stimulating immune and antigen presentation in piglets’ response and physiological changes to the infection of E.coli F18. |
| Keywords: | swine SLA-DQA gene immune response Escherichia coli F18 |
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