| 1株山羊内源性肺腺瘤反转录病毒的序列测定与分析 |
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| 引用本文： | 周艳喜,李靖,敖威华,刘霄卉,于立新,幺宏强,马学恩.1株山羊内源性肺腺瘤反转录病毒的序列测定与分析[J].畜牧兽医学报,2012,43(5):838-842. |
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| 作者姓名： | 周艳喜 李靖 敖威华 刘霄卉 于立新 幺宏强 马学恩 |
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| 作者单位： | 1. 内蒙古农业大学兽医学院,呼和浩特,010018
2. 内蒙古出入境检验检疫局,呼和浩特,010020 |
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| 基金项目： | 国家自然科学基金资助项目 |
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| 摘 要： | 参照内源性绵羊肺腺瘤病毒株enJSRV-20(EF680302.1)全基因组序列设计合成3对引物,以山羊基因组DNA为模板,应用PCR技术扩增山羊内源性病毒基因片段,分别连接PMD-18T载体并测序,完成了1株山羊内源性肺腺瘤反转录病毒全基因序列的测定。结果分析显示测定序列全长7 480bp,获得的序列与内源性绵羊肺腺瘤病毒株enJSRV-18(EF680301.1)的核苷酸相似性为93.4%,与外源性绵羊肺腺瘤病毒株JS-7(AF357971.1)的核苷酸相似性为88.1%。根据pro基因序列构建系统进化树,结果显示扩增序列与内源性绵羊反转录病毒株en-JSRV-5(EF680305.1)的亲缘关系最近。本研究有助于内源性肺腺瘤反转录病毒感染性克隆的构建和内源性肺腺瘤反转录病毒功能的研究。
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| 关 键 词： | 山羊内源性肺腺瘤反转录病毒 全基因序列 PCR扩增 |
Cloning and Sequence Analysis of Goat's Endogenous Pulmonary Adenomatosis Retrovirus |
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| ZHOU Yan-xi
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LI Jing
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AO Wei-hua
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LIU Xiao-hui
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YU Li-xin
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YAO Hong-qiang
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MA Xue-en.Cloning and Sequence Analysis of Goat's Endogenous Pulmonary Adenomatosis Retrovirus[J].Acta Veterinaria et Zootechnica Sinica,2012,43(5):838-842. |
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| Authors: | ZHOU Yan-xi LI Jing AO Wei-hua LIU Xiao-hui YU Li-xin YAO Hong-qiang MA Xue-en |
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| Institution: | 1 (1.College of Veterinary Medicine,Inner Mongolia Agricultural University,Huhhot 010018,China; 2.Inner Mongolia Enty-Exit Inspection and Quarantine Bureau,Huhhot 010020,China) |
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| Abstract: | Three pairs of primers were designed according to the sequence of strain enJSRV-20.Sequence of goat’s enJSRV was amplified by PCR technology and these PCR products were cloned into PMD18-T vector and then sequenced.The complete genome of goat’s enJSRV was obtained successfully.Analysis results showed that the sequence of goat’s enJSRV was 93.4% similarity with enJSRV-18 and 88.1% similarity with strain JS-7,respectively.Phylogenetic analysis according to pro gene showed that goat’s enJSRV had higher homology with strain enJSRV-5.It will be useful for the study of constructing infectious clone and elucidation the function of endogenous pulmonary adenomatosis retrovirus. |
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| Keywords: | goat’s enJSRV full-length genome sequence PCR amplification |
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