| 异源抗体对接方法预测流感病毒血凝素B细胞构象表位 |
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| 引用本文： | 梁瑾,王靖飞,吴春艳,冯宝龙.异源抗体对接方法预测流感病毒血凝素B细胞构象表位[J].中国预防兽医学报,2010,32(1). |
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| 作者姓名： | 梁瑾 王靖飞 吴春艳 冯宝龙 |
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| 作者单位： | 1. 中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室,黑龙江,哈尔滨,150001
2. 东北农业大学继续教育中心,黑龙江,哈尔滨,150030 |
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| 摘 要： | 为了对异源抗体对接方法预测流感病毒血凝素B细胞构象表位的可行性进行分析,本研究基于蛋白质相互作用界面的形状互补理论,使用异源抗体(来自于抗原抗体复合物1G9M、1RVF、1TPX、1NCA、1A2Y)的晶体结构同流感病毒血凝素(A/Aichi/2/68(H3N2))的晶体结构进行刚性分子对接,通过计算B细胞表位的预测表面和实际表面C-α原子之间的RSMD以及表位氨基酸的准确率,分析用异源抗体探测出血凝素B细胞表位的可能性。结果表明,不同的异源抗体均可结合到A/Aichi/2/68(H3N2)的已知B细胞表位,最佳的对接构象与原晶体结构重叠后表位残基C-α原子的均方根偏差均小于0.6埃,表位氨基酸预测的准确性大于60%,说明异源抗体对接方法可以尝试用于B细胞构象表位的预测。
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| 关 键 词： | 构象表位预测 异源抗体 分子对接 |
The feasibility analysis of using heterogenous antibody docking for predicting the B cell epitope of influenza virus hemagglutinin |
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| LIANG Jin,WANG Jing-fei,WU Chun-yan,FENG Bao-long.The feasibility analysis of using heterogenous antibody docking for predicting the B cell epitope of influenza virus hemagglutinin[J].Chinese Journal of Preventive Veterinary Medicine,2010,32(1). |
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| Authors: | LIANG Jin WANG Jing-fei WU Chun-yan FENG Bao-long |
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| Institution: | LIANG Jin1,WANG Jing-fei1,WU Chun-yan1,FENG Bao-long2 (1.State Key Laboratory of Veterinary Biotechnology,Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Harbin 150001,China,2.The Centre of Proceeding Education,Northeast Agricultural University,Harbin 150030,China) |
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| Abstract: | The influenza virus hernagglutinin (A/Aichi/2/68 (H3N2)) and the the non-influenza virus hemagglutinin antibody (from the antgen-anfibody complexes) were docked based on the shape complementarities at protein-protein interfaces, and the possibility of using heterogenous antibody to detect the binding surface of the hemagglutinin antibody are analysed. The results showed that the C-α atoms RMSD calculation of interface residue was below 0.6 ℃ after superimposition, and >60 % residues at the antibody-binding site could be predicted. This method could be useful for prediction of B cell discontinuous epitopes. |
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| Keywords: | discontinuous epitopes prediction heterogenous antibody molecular docking |
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