An Efficient Inoculation Method for Colonization of Chinese Cabbage Seedlings by the Root Endophytic Fungus Heteroconium chaetospira |
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Authors: | Fumiaki USUKI Kazuhiko NARISAWA Miho YONEZAWA Makoto KAKISHIMA Teruyoshi HASHIBA |
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Institution: | (1) Institute of Agriculture and Forestry, University of Tsukuba, Tsukuba 305–8572, Japan, JP;(2) Plant Biotechnology Institute, Ibaraki Agricultural Center, Ibaraki 319–0292, Japan, JP;(3) Department of Environment Biotechnology, Graduate school of Agriculture, Tohoku University, Sendai 981–8555, Japan, JP |
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Abstract: | To develop an efficient method to inoculate Chinese cabbage seedlings with the root endophytic fungus Heteroconium chaetospira, an appropriate nursery soil and glucose concentration for the nutrient medium for fungal colonization were determined. A grid-sheet
method was established for estimating the degree of colonization of entire roots by the fungus. The fungus colonized at high
frequencies when peat moss was used as the rooting medium. Colonization was highest (75%) when peat moss was amended with
0.1% glucose. Under these conditions, fungal hyphae developed intracellularly in root cortical cells. In contrast, under high
glucose conditions, fungal colonization was restricted mostly to intercellular regions of epidermal or cortical root tissues.
Here, hyphae formed inter- or intracellular microsclerotia.
Received 19 August 2002/ Accepted in revised form 5 December 2002 |
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Keywords: | : Heteroconium chaetospira root endophyte Chinese cabbage inoculation method root colonization |
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