| 草莓原生质体的培养和植株再生 |
| |
| 引用本文： | 吕长平,郑玉生,石雪晖,张秋明,杨国顺,邓建平.草莓原生质体的培养和植株再生[J].江西农业大学学报,2003,25(2):254-257. |
| |
| 作者姓名： | 吕长平 郑玉生 石雪晖 张秋明 杨国顺 邓建平 |
| |
| 作者单位： | 1. 湖南农业大学,园艺园林学院,湖南,长沙,410128
2. 长沙市农业学校,湖南,长沙,410127 |
| |
| 摘 要： | 通过对已分离提纯的草莓原生质体的培养研究 ,比较得出以KM为培养基 ,以 0 .4mol L葡萄糖和 0 .1mol L蔗糖或仅以 0 .5mol L葡萄糖作碳源 ,采用低溶点琼脂糖包埋的培养方式 ,培养密度采用 1× 10 6个 mL ,原生质体出现分裂和小愈伤组织形成的时间都较早。以MS1 、MS2 、MS3 为培养基、采用分步诱导的方法 ,能顺利诱导产生新植株 ,并在MS培养基上生根良好
|
| 关 键 词： | 草莓 原生质体培养 植株再生 培养基 |
| 文章编号： | 1000-2286(2003)02-0254-04 |
| 修稿时间： | 2003年2月25日 |
Protoplast Culture and Plant Regeneration of Strawberry |
| |
| Abstract: | Through the contrast studies of protoplast culture, it was found that the best condition included KM medium 0.4 mol/L glucose and 0.1mol/L sucrose or only 0.5mol/L glucose as carbon hydrate source, the method of LMET embedding. In this condition, both time of the first division of protoplast and microcalli formation were earlier, plant-lets from protoplast-derived callus were regenerated by using MS 1, MS 2 and MS 3 as media and steps differentiation method. It was easy for the plant-lets to root on MS media. |
| |
| Keywords: | strawberry protoplast culture plant regeneration |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
