磨盘柿离体叶片愈伤组织发生及不定芽诱导

研究了培养基种类、植物生长调节剂配比、叶片不同部位及培养条件对磨盘柿离体叶片愈伤组织发生及不定芽再生的影响。适宜磨盘柿叶片再生的基本培养基为改良MS(1／2 N),生长调节剂为ZT 4.0~6.0 mg/L或TDZ 1.0mg／L与IAA 0.1 mg／L组合,不宜用BA和高浓度的NAA;ZT和TDZ促进叶片再生的方式不同,前者利于直接再生,后者需降低细胞分裂素浓度,二次诱导使芽点生长成苗;叶片接种后暗培养2~3周再生效率高;叶片不同部位中以叶基部最易再生,其次为叶中部,叶尖最差。

Callus Formation and Adventitious Bud Regeneration From Leaves in vitro in Persimmon (Diospyros kaki)

The effects of basic media, plant growth regulators and their concentration, and parts of leave on callus formation and adventitious bud regeneration were studied in persimmon of Mopanshi (Diospyros kaki Thunb). The results showed that modified MS medium supplied with 4.0 mg/L ZT (or 1.0 mg/L TDZ) and 0.1 mg/L IAA was the optimal protocol for adventitious bud regeneration. It was not suggested to use BA and high concentration NAA. The effects of ZT and TDZ on the generation from leaf disks were different. Applied ZT could benefit to the generation directly. But the leaf disks with TDZ treatments need to be transferred into the medium supplied with lower concentration of cytokinin for further culture to allow the adventitious bud growth. Dark incubation for two to three weeks could increase the percentage of adventitious bud regeneration. Leaf disks from near the petioles were the easiest to generate adventitious buds, followed by that from the middle of leaves and the leaf tips.