木质素代谢途径相关酚酸和类黄酮的HPLC测定方法的研究

为了研究木质素代谢途径中相关酚酸和类黄酮的测定方法。采用有机试剂萃取和超声破碎细胞的方法对烟草总酚酸和类黄酮进行提取，并通过高效液相色谱（HPLC）对烟草中6种植物酚酸和5种植物类黄酮进行了定量分析。结果显示：有机萃取结合超声破碎细胞的方法能够将酚酸和类黄酮从植物中有效分离；高效液相色谱（HPLC）能够将6种植物酚酸和5种植物类黄酮一次分离；HPLC测定香豆酸、阿魏酸、芥子酸、对甲氧基苯甲酸和肉桂酸的保留时间分别为18.11、20.03、20.51、20.76、22.86、26.23；HPLC测定杨梅素，槲皮素，根皮素，柑橘素和山奈酚的保留时间分别为9.06、12.01、14..96、15.23、16.12。通过研究表明，HPLC能够很好的对植物酚酸和类黄酮进行检测，这为研究木质素代谢途径相关酚酸和类黄酮奠定了基础。

HPLC Measure the Phenolic acid and Flavonoid Relate to the Lignin Biosynthesis

The aim was to establish a research method to measure the phenolic and flavonoid which relate to plant lignin biosynthesis. Organic reagent and ultrasonication were used for plant phenolic and flavonoid extract, HPLC was used for the measurement of six plant phenolic and five flavonoid. Tobacco was used as sample to test the HPLC measure method, the result show that HPLC method for the phenolic and flavonoid relate to plant lignin biosynthesis is efficient which can isolate 6 different phenolics and 5 different flavonoids in one test；organic reagent and ultrasonication are useful to extract plant phenolic and flavonoid; the retention time of p-Coumaric, Ferulic, Caffeic, Cinnamic, Sinapic and 4-Methoxybenzoic Acid are 18.11, 20.03, 20.51, 20.76, 22.86 and 26.23; the retention time of Myricetin, quercetin, phloretin, naringenin and kaempferol are 9.06, 12.01, 14..96, 15.23, 16.12. The method made foundation for the research of plant lignin biosynthesis.