木薯渣、桉树渣固体发酵Bacillus amyloliquefaciens WJ22研制生物有机肥的配方研究

Bacillus amyloliquefaciens WJ22为实验室保存的香蕉根际促生功能菌。利用牛粪有机肥和菜籽饼为基本原料，海南当地农业废弃物木薯渣和桉树皮为添加原料，研究该菌株的固体发酵配方工艺，最终获得利用该菌株研制的促生生物有机肥。结果表明，拮抗菌WJ22在菜籽饼含量为15%、木薯渣含量为20%、桉树渣含量为20%的原料混合物中固体发酵效果最佳，菌株数量超过2×109 cfu/(g·干重)，远远超过国家固体生物有机肥的标准[2×107 cfu/(g·干重)]。盆栽试验结果表明，该配方研制的生物有机肥能够很好地促进香蕉苗期的生长。

Solid fermentation ingredient of Bacillus amyloliquefaciens WJ22 to produce bio-organic fertilizer by cassava residue and eucalyptus slag

A rhizosphere bacteria-Bacillus amyloliquefaciens WJ22 stored in our laboratory was a plant growth-promoting rhizosphere bacterium. In the present study, ripe cow manure compost and rapeseed cake as raw materials, cassava residue and eucalyptus slag as addition materials were chosen from agriculture wastes in Hainan province to solid fermented strain WJ22 and the bio-organic fertilizer made from this strain was prepared. The results showed that strain WJ22 could grow well in the mixture contain 65% of ripe cow manure compost, 15% of rapeseed cake, 20% of cassava residue, and 20% of eucalyptus slag. The strain number is more than 9 × 108 cfu/g dry weight at the ending of composting process and were far more than the national solid bio-organic fertilizer standard (2 × 107 cfu/g dry weight). Pot experiment results showed that the bio-organic fertilizer made from WJ22 could efficiently promote the growth of banana.