蔷薇科植物DELLA蛋白的生物信息学分析

为探索蔷薇科植物DELLA蛋白的结构特征和亲缘进化关系，以蔷薇科植物苹果、梨和玫瑰等19个DELLA蛋白为试材，利用expasy、PSORT和PROSITE数据库、TM-HMM方法、SignalP4.0Server、CDD、DNAMAN 和MEGA version5.3等软件对蛋白质进行了生物信息学分析。结果表明：蔷薇科植物中19个DELLA蛋白氨基酸序列组成成分和理化性质差异不明显，均为非跨膜类亲水性蛋白，且不含信号肽；不同DELLA蛋白之间同源性较高，达到74.38%以上，具有DELLA和GRAS 2个保守结构域，功能位点是GRAS；19个DELLA蛋白N端同源性较低，但是存在TVHYNP、VHIID和RVER等DELLA蛋白典型结构域；进化树显示梨属和蔷薇属属内植物DELLA蛋白亲缘关系较近，苹果属内植物亲缘关系相差较远。本研究开展为蔷薇科植物遗传演化研究提供理论依据。

A bioinformatic analysis of DELLA protein in Rosaceous plants

In order to explore the structural feature and phylogenetic analysis of DELLA protein in Rosaceous plants, 19 DELLA proteins of Malus, Pyrus and Rose in Rosaceous plants were analyzed by using expasy, PSORT and PROSITE date bank, TM-HMM, SignalP4.0Server, CDD, DNAMAN and MEGA version5.3 softwares. The results showed that all DELLA proteins were non-transmembrane hydrophilic proteins and without the signal peptides, and the difference was insignificant among their amino acid composition, physical and chemical characteristics. There was high homology among the different DELLA proteins, reached above 74.38%. Both DELLA and GRAS have two conserved domains, and the function sites were GRAS. The homology of the N-terminal of the 19 DELLA proteins were low, however they had the DELLA protein typical domains such as TVHYNP, VHIID and RVER etc. Phylogenetic tree analysis showed DELLA proteins have the closest relationships in Pyrus and Rose plants, and the distant relationships in Malus plants. The study provided theoretical basis for the genetic evolution of Rosaceous plants.