扁吻鱼石蜡切片制作方法的探讨

为探讨新疆扁吻鱼石蜡切片的制作方法，观察新疆扁吻鱼的基本组织结构，总结切片制作过程中出现的问题，分析问题产生的原因和解决办法。利用组织学石蜡切片技术和苏木精-伊红染色技术，用电子显微镜观察扁吻鱼各个组织结构。结果显示：新疆扁吻鱼的鳃丝由两排鳃片组成，每根鳃丝的两侧又着生许多细小的鳃小片，染色后可看见出鳃动脉和入鳃动脉，扁吻鱼的肌肉切片要比鳃切片更清晰，可看见肌纤丝和结缔组织间隙毛细血管的存在，扁吻鱼的肝脏细胞较大，核仁大而明显，细胞排列紧密，本实验对新疆扁吻鱼的鳃、肌肉和肝脏的显微结构有了初步的认识，为新疆扁吻鱼的研究提供了切实可靠的组织学参考依据。

The Exploring of Paraffin Sections Manufacture Methods of Aspiorhynchus laticeps

In order to investigate the method of making Aspiorhynchus laticeps paraffin section,understanding its basic structure and summarizing the problems in the production of paraffin section, analyzed the reasons and solutions. It was a good way for using the technique of histological paraffin sectioning and Hematoxylin-eosin staining to observe the organizational structure of Aspiorhynchus laticeps. The results showed that there were two rows of gill lamellae, each side with a lot of small gill lamella, each gill filaments have small gill lamellae, afferent branchial artery and efferent branchial artery were visible after staining, the muscle slice was more clearly than gills slice of Aspiorhynchus laticeps. The presence of capillary was clear between the muscle fibrils and connective tissue of Aspiorhynchus laticeps. The nucleoli of liver cells was larger than the other nucleoli, all of the cell was closely packed. From the experiment we could got a preliminary understanding about the gill, muscle and liver of Aspiorhynchus laticeps, it was provided a reliable histological basis data for the research of Aspiorhynchus laticeps.