新疆几种野生禾草和草坪禾草的内生真菌带菌率检测

本研究采用苯胺蓝染色法对新疆部分野生禾本科牧草及草坪牧草的内生菌带菌率进行了检测。结果表明，同一地理位置不同种属牧草以及不同地理位置同一种属牧草的带菌率存在显著差异。披碱草叶鞘和种子的带菌率均高达58%。绝大部分禾草的叶鞘内未检测到内生菌，而在其种子的糊粉层中却普遍发现了菌丝，表明部分禾本科植物的内生菌并非系统感染。

Microscopic Detection of Endophytic Fungus Carrying Rate from Wild and Lawn Gramineous Grasses in Xinjiang

The interaction of plant-endophytic association was very significant for the ecological adaptability of the grasses in arid region. This research aimed at the endophytic fungi carrying rate and their morphology through microscopically inspecting the different parts of several gramineous grasses collected in Xinjiang, which could provide information and material for the future application of such endophytic fungal resources. Aniline blue staining method was applied for detection of endophytic fungi carrying rate in the seed skin and aleuronic layer and leaf sheaths. The result showed that leaf sheaths, aleurone layer, seed coat of Elymus was detected in mycelia, and carrier rate was high; the vast majority of respondents were not detected in leaf sheath of endophytic bacteria; many plant seed aleurone layer was not detected in endophytic bacteria; however the belt strain rate was very high, most of them could be detected and hyphae; Xinjiang gramineous grasses had many symbiotic bacteria, and provided rich resources for the physiological and biochemical characteristics of host and endophyte.