中蜂囊状幼虫病病毒检测及地区间遗传变异

为了摸清云南蒙自地区整个中蜂场春繁季节中蜂囊状幼虫病病毒(CSBV)的感染及其病毒株分化情况。运用RT-PCR对57群滇南中蜂进行CSBV带毒率检测和遗传变异分析。结果表明：该蜂场有26群滇南中蜂感染CSBV，感染率为45.6%，其中隐性感染为31.57%，显性感染为14.04%。所选用的2对引物均能较好地对滇南中蜂CSBV进行分子识别鉴定，其中第二对引物更适合；种群间RNA序列变异分析表明：云南种群与重庆种群聚为一个分支，遗传距离为0.014。说明春繁季节是云南蒙自地区滇南中蜂CSBV发生的重要时段，应提早采取预防措施；CSBV病毒株在不同地区存在分化现象，云南种群与重庆种群遗传关系较近，推测CSBV地理距离与遗传距离可能有关系。

Detection and Hereditary Variation among Regions of Chinese Sacbrood Virus

In order to find out the infectious status and the strains differentiation of CSBV in an Apis cerana apiary in spring season in Mengzi, Yunnan Province. The detection of the infection rate and the hereditary variation of CSBV for 57 Apis cerana austro-yunnanensis colonies by RT-PCR. The results showed that: 26 colonies were infected with CSBV in the apiary, and the infection rate was 45.6%. The occult infection rate was 31.57%, and the apparent infection rate was 14.04%. The two pairs of primers could identificate CSBV in Apis cerana austro-yunnanensis, and the second pair was better. RNA sequence variation among the populations was analysed, which displayed that Yunnan population and Chongqing population clustered a branch, and the genetic distance was 0.014. Spring season were important period for occurrence of CSBV in Mengzi, Yunnan Province. It should be taken early precautions. CSBV strains existed variation phenomenon in different regions. The genetic relationship between Yunnan population and Chongqing population was close. It was hypothesized that CSBV geographic distance and genetic distance might have a relationship.