Size, shape and surface morphology of starch granules from Norway spruce needles revealed by transmission electron microscopy and atomic force microscopy: effects of elevated CO(2) concentration

We compared the effects of ambient (350 ppm) and elevated CO(2) concentration (700 ppm) on the size and shape of starch granules in Norway spruce (Picea abies (L.) Karst.) needles during one growing season. Starch granules were isolated from needles by alkaline digestion and analyzed by transmission electron microscopy (TEM) and atomic force microscopy (AFM). Measurements made with a particle size analyzer indicated that starch granules ranged between 0.5 and 10 microm. Granule size and shape varied according to needle developmental stage and CO(2) concentration. Generally, elevated CO(2) concentration increased the size of the starch granules. Fine surface structures (< 10 nm in size) studied by AFM were characterized by the presence of protrusions, furrows and pores.