| HPLC/ESI—MS法测定槟榔中的槟榔碱 |
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| 引用本文： | 丁芳林,彭书练.HPLC/ESI—MS法测定槟榔中的槟榔碱[J].农产品加工．学刊,2008(4):76-79. |
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| 作者姓名： | 丁芳林 彭书练 |
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| 作者单位： | 1. 湖南生物机电职业技术学院,湖南,长沙,410127
2. 湖南省农产品加工研究所,湖南,长沙,410125 |
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| 摘 要： | 建立槟榔中槟榔碱的高效液相色谱—电喷雾质谱(HPLC—ESI—MS)检测方法。通过优化色谱和质谱条件,以β-蒎烯为内标物,利用质谱定性和色谱定量测定槟榔碱的含量。槟榔碱在质量浓度为10.8￣86.4μg/mL时的线性关系良好,R=0.9985;最低检测限为0.4μg/mL(S/N=3);最低定量限为0.9μg/mL(S/N=10);平均回收率为98.32%,RSD为3.44%(n=5)。该方法具有流动相简单、分析时间短,以及不需对样品进行衍生,操作简便、定量准确和抗干扰能力强等优点。
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| 关 键 词： | 槟榔 槟榔碱 高效液相色谱—电喷雾质谱 |
| 文章编号： | 1671-9646(2008)04-0076-04 |
| 修稿时间： | 2008年3月10日 |
Determination of Arecoline from Areca Nut by High Performance Liquid Chromatography/mass Spectrometry |
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| Ding Fanglin,Peng Shulian.Determination of Arecoline from Areca Nut by High Performance Liquid Chromatography/mass Spectrometry[J].Nongchanpin Jlagong.Xuekan,2008(4):76-79. |
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| Authors: | Ding Fanglin Peng Shulian |
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| Institution: | Ding Fanglin1,Peng Shulian2(1. Hu\'nan Biological , Electromechanical Polytechnic,Changsha,Hu\'nan 410127,China,2. Hu\'nan Agricultural Product Processing Institute,Hu\'nan 410125,China) |
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| Abstract: | Objective:To develop a high performance liquid chromatography-electrospray mass spectrometry(HPLC-ES-MS)method to determine arecoline.Methods:The internal standard was C10H16. Arecoline was identified by electrospray ionization mass spectrometry and quantified by high performance liquid chromatography.Results:The calibration curves were linear in the ranges 10.8~86.4μg/mL for Arecoline(R=0.998 5),The average recoveries was 98.32%,RSD=3.44%(n=5).Determination minimum was 0.4 μg/mL(S/N=3). Quantifition minimum was 0.9 μg/mL(S/N=10). The average recovery of the method was 98.32%,RSD=3.44%(n=5). Condusion:The proposed method had the advantages of easy operation,without derivation,less analysis time,good precision and accuracy. |
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| Keywords: | areca nut arecoline high performance liquid chromatography/mass spectrometry |
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