A method for aseptic culture of bud explants from citrus trees |
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| Authors: | I Giladi A Altman R Goren |
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| Institution: | Department of Horticulture, The Hebrew University of Jerusalem, Faculty of Agriculture, Rehovot, Israel |
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| Abstract: | Culture of citrus bud explants sampled from outdoor trees is sometimes difficult because of contamination not overcome by standard surface-sterilization. An improved method, involving a 5-minute immersion of explants in 80% (v/v) ethanol, which does not interfere with normal explant and callus development, is described in detail. |
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