| 巨桉林下马勃分子系统发育的关系 |
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| 引用本文： | 潘欣,张健,朱天辉,梁洪萍.巨桉林下马勃分子系统发育的关系[J].林业科学,2011,47(5). |
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| 作者姓名： | 潘欣 张健 朱天辉 梁洪萍 |
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| 作者单位： | 1. 四川农业大学林学院,雅安625014;成都理工大学地球科学学院,成都610059
2. 四川农业大学林学院,雅安,625014 |
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| 基金项目： | 国家"十一五"科技支撑计划项目，国家自然科学基金项目 |
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| 摘 要： | 采集巨桉林下马勃子实体,培养其菌丝体,提取子实体及菌丝体基因组DNA,进行rDNA-ITS区序列的扩增、克隆和序列测定,并对rDNA-ITS不同区域作序列分析,首次构建马勃的系统发育树.结果表明:野外采集获得马勃子实体l0种,其中成功培养6种,测序结果表明马勃rDNA-ITS区长度在607～766 bp之间,系统发育分析表明硬皮马勃属(Scleroderma)与豆包菌(Pisolithus)亲缘关系较近,秃马勃属(Calvatia)、马勃属(Lycoperdon)及横膜马勃属(Vascellum)之间亲缘关系较近,ITSl-5.8S rDNA-ITS2区可建立马勃类真菌属间系统发育树,ITS2区可用于建立马勃类真菌属内系统发育树,3个待定种硬皮马勃Scleroderma sp.11-1,Scleroderma sp.2-2和Scleroderma sp.5-2为金黄硬皮马勃(S.aurantium)的可能性较大.此研究可为探讨巨桉人工林下外生菌根种类与作用机制、马勃分类系统学及菌丝体的开发研究奠定基础.
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| 关 键 词： | 巨桉林 马勃 系统发育 ITS序列 四川 |
Analysis of Puffballs' Phylogenetic Relationships from Eucalyptus grandis Forest |
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| Pan Xin,Zhang Jian,Zhu Tianhui,Liang Hongping.Analysis of Puffballs' Phylogenetic Relationships from Eucalyptus grandis Forest[J].Scientia Silvae Sinicae,2011,47(5). |
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| Authors: | Pan Xin Zhang Jian Zhu Tianhui Liang Hongping |
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| Abstract: | Mycelia were cultivated from a fruiting body of puffballs which were collected from the Eucalyptus grandis forest,and the genomic DNA was extracted as template.Subsequently,the internal transcribed spacer(ITS) region in rDNA gene was amplified,cloned and sequenced.The phylogenetic tree of puffballs was first built based on different part of ITS region.The results showed that six species of puffballs were cultivated successfully using a PDA medium after comparing the sequences of puffballs fruiting body and ... |
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| Keywords: | Eucalyptus grandis forest puffballs phylogenetic relationships ITS region Sichuan Province |
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