枸杞叶片DNA不同提取方法比较 |
| |
引用本文: | 思彬彬,尚洁,马艳辉. 枸杞叶片DNA不同提取方法比较[J]. 安徽农业科学, 2009, 37(15): 6872-6872 |
| |
作者姓名: | 思彬彬 尚洁 马艳辉 |
| |
作者单位: | 北方民族大学生命科学与工程学院,宁夏,银川,750021;北方民族大学生命科学与工程学院,宁夏,银川,750021;北方民族大学生命科学与工程学院,宁夏,银川,750021 |
| |
摘 要: | [目的]为研究枸杞的遗传多样性、种质鉴定和指纹图谱的构建提供基础保证。[方法]以枸杞成熟叶片为试材,分别采用常规CTAB法、高盐CrAB法等11种方法从枸杞中提取基因组DNA,比较不同方法的提取效果。[结果]11种方法中,除5XCTAB法试验结果不理想外,其余10种均能有效地从枸杞叶片中获得DNA,所提DNA的质量和产量均无明显差异。[结论]11种方法提取的枸杞基因组DNA,能够满足RAPD分析的需要。
|
关 键 词: | 枸杞 成熟叶片 DNA提取 |
Comparison of Different Extraction Methods of Genomic DNA from the Leaves of Lycium barbarum L. |
| |
Affiliation: | SI Bin-bin et al (College of Life Science and Enghaeering,Northern University for Natio'nalities,Yinchuan,Ningxia 750321) |
| |
Abstract: | [Objective] The research aimed to provide basic guarantee for studying the genetic diversity the germplase identification and construction of fingerprint of Lycium barbarum L.[Method] With mature leaves of Lycium barbarum L.as tested material,the genomic DNA was extracted from Lycium barbarum L.using eleven different methods,such as CTAB method,high-salt CTAB method and so on.The extraction effects of different methods were compared.[Result] High-quality DNA could be obtained with ten of all methods,except 5×CTAB method.Quality and outtput of DNA had no significant differences.[Conclusion]The genomic DNA could be extracted from the mature leaves of Lycium barbarum L.by 11 kinds of methods.These methods all could be used in RAPD reaction. |
| |
Keywords: | Lycium barbarum L. Mature leaves DNA extraction |
本文献已被 维普 万方数据 等数据库收录! |
|