基因分析实现CAV VP1基因在大肠杆菌中的高效表达

通过分子生物学软件DNASTAR对CAV VP1的基因分析发现,CAV VP1基因的5端非抗原区编码蛋白的密码子中存在连续的大肠杆菌稀有密码子。为了在大肠杆菌中高效表达CAV VP1,文章研究扩增了不含5’端稀有密码子的CAV VP1基因,并将其插入原核表达载体pGEX-4T-1,构建了重组表达载体pGEX-VP1,成功进行了高效表达。电泳条带分析表明,融合蛋白的表达量在44%左右,为研究CAV VP1的抗原性提供了丰富的来源。

High Expression of VP1 gene of Chicken Anemia Virus in E.coli by analysis to gene

By analysis of VP1 gene of chicken anemia virus by DNASTAR software,it is recovered there are a lot of rare codons in 5' end of VP1.For expressing effectively VP1 in E.coli,the gene of VP1 without 5' end was increased and inserted into expressing vector pGEX-4T-1 to form recombinant plasmid pGEX-VP1.The recombinant fusion protein was highly expressed in E.coli BL21.The quantity of fusion protein is up to 44% of bacterium protein.The success to high expression CAV VP1 is helpful to research for the antigenic activity of CAV VP1.