| 培养液中不同浓度肝素对蓝狐精子体外获能的影响 |
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| 引用本文： | 白玉妍,郭婷婷,刘志平.培养液中不同浓度肝素对蓝狐精子体外获能的影响[J].经济动物学报,2011,15(2):63-66. |
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| 作者姓名： | 白玉妍 郭婷婷 刘志平 |
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| 作者单位： | 1. 东北林业大学野生动物资源学院,哈尔滨,150040
2. 东北林业大学机电工程学院,哈尔滨,150040 |
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| 基金项目： | 中央高校基本科研业务专项资金项目(DL09BA38) |
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| 摘 要： | 采用上游法分离优化精子,以mTyrod's液作为基础培养液检测培养液中不同质量浓度肝素对蓝狐精子体外获能的影响,试验设4个肝素质量浓度:0,10,20,50 μg/mL,38.5℃、5%C02培养箱进行孵育,获能培养时间为6h.利用考马斯亮蓝染色法检测精子顶体反应率,伊红-苯胺黑染色法检测活精子比率,观测法检测精子活力...
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| 关 键 词： | 蓝狐 精子 精子获能 肝素 |
Different Concentrations of Heparin on Capacitation of Alopex lagopus Sperm in Vitro |
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| BAI Yu-yan,Guo Ting-ting,LIU Zhi-ping.Different Concentrations of Heparin on Capacitation of Alopex lagopus Sperm in Vitro[J].Journal of Economic Animal,2011,15(2):63-66. |
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| Authors: | BAI Yu-yan Guo Ting-ting LIU Zhi-ping |
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| Institution: | BAI Yu-yan1,Guo Ting-ting2,LIU Zhi-ping1(1.College of Wildlife Resources,Northeast Forestry University,Harbin 150040,China,2.College of Mechanical and Electrical Engineering,China) |
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| Abstract: | In order to evaluate different concentrations of heparin on capacitation of Alopex lagopus sperm in vitro,the swim-up method was used for selecting sperms,and mTyrod's solution was the basic nutrient solution.Different concentrations(0,10,20,50 μg/mL)of heparin was added to mTyrod's solution respectively at 38.5 ℃ in 5% CO2 incubator.The incubation time was 6 hours.The acrosome reaction rate was determined by coomassie blue staining and sperm viability was determined by eosin-nigrosine staining.Sperm activity was observed at 0,1,2,4 and 6 hour.The result showed that heparin could induce capacitation of Alopex lagopus sperm in vitro,and the suitable consistence of the heparin was 20 μg/mL. |
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| Keywords: | blue fox sperm capacitation in vitro heparin〖HT |
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