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Isolation of two strains of a new Tombusvirus (Havel river virus, HaRV) from surface waters in Germany
Authors:R Koenig  E Pfeilstetter  H Kegler  DE Lesemann
Institution:(1) Biologische Bundesanstalt für Land-und Forstwirtschaft (BBA), Institut für Pflanzenvirologie, Mikrobiologie und biologische Sicherheit, D38104 Braunschweig, Germany;(2) BBA, Abteilung für nationale und internationale Angelegenheiten der Pflanzengesundheit, D06449 Aschersleben, Germany;(3) Bäckersteig 11, D06449 Aschersleben, Germany
Abstract:Two virus isolates from water samples — one from a small stream in South Western Germany and another one from the Havel river in North Eastern Germany c. 500 km away, proved to be strains, named S and H, respectively, of a new Tombusvirus for which the name Havel river virus (HaRV) had been suggested previously in a brief account. Immunoelectron microscopical decoration tests and sequence comparisons of the coat proteins indicated that the two HaRV strains are only distantly related to known Tombusviruses. The closest relationships were found to Cucumber necrosis virus. Nothing is known about their natural hosts. Because the S strain of HaRV was isolated in a woody area from a small stream close to its origin, they may be pathogens of trees or wild plants in such habitats.
Keywords:comparisons of Tombusvirus coat protein sequences  immunoelectron microscopy  Tombusvirus relationships  viruses in surface waters
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